Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues

doi:10.1166/sam.2015.2178

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	Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues
	Zhang, Dianbao 1,2; Wang, Jing 3; Wang, Zhe 4; Wang, Rui 1,2; Song, Lina 5; Zhang, Tao 1,2; Lin, Xuewen 1,2; Shi, Ping 6; Xin, Hongchuan 7; Pang, Xining 1,2
	2015-06-01
发表期刊	SCIENCE OF ADVANCED MATERIALS
卷号	7 期号:6 页码:1058-1064
摘要	Mesenchymal stem cells (MSCs) are emerging as a promising therapeutic approach and genetic modification by non-viral vectors could span their applications. Herein, the capacity of PEI-coated Fe3O4 nanoparticles (NPs) for siRNA delivery to human MSCs derived from different tissues was evaluated. The prepared spherical NPs with charge of around +44.0 mV and an average diameter of 12 nm exhibited proper siRNA encapsulation and enhanced serum stability. High-purity human MSCs were isolated from bone marrow, adipose tissue and amnion membrane, and GAPDH siRNA and siRNA NC (negative control) was transfected into MSCs using NPs prepared. When 4 ng/ml NPs were used at NPs/siRNA weight ratio of 4, high transfection efficiency and low toxicity were observed and silencing efficiencies were higher than 60%. Additionally, the in vitro tests indicated that the NPs entered into MSCs by endocytosis. According to those results, PEI-coated Fe3O4 NPs possessed potential as a safe and effective siRNA delivery reagent for MSCs and may be fit for stem cell therapy.
文章类型	Article
关键词	Pei-coated Fe3o4 Nanoparticles Mesenchymal Stem Cells Sirna Delivery Endocytosis
WOS标题词	Science & Technology ; Technology ; Physical Sciences
DOI	10.1166/sam.2015.2178
关键词[WOS]	IRON-OXIDE NANOPARTICLES ; GENE DELIVERY ; MEDICINE ; TRANSFECTION ; THERAPY ; DISEASE
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS记录号	WOS:000352332800007
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6423
专题	科技处
作者单位	1.China Med Univ, Minist Publ Hlth, Key Lab Cell Biol, Dept Stem Cells & Regenerat Med, Shenyang 110001, Peoples R China 2.China Med Univ, Minist Educ, Key Lab Med Cell Biol, Shenyang 110001, Peoples R China 3.China Med Univ, Affiliated Hosp 1, Dept Anus & Intestine Surg, Shenyang 110001, Peoples R China 4.China Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China 5.Southeast Univ, Sch Biol Sci & Med Engn, Jiangsu Lab Biomat & Devices, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China 6.China Med Univ, Affiliated Hosp 1, Dept Gen Practice, Shenyang 110001, Peoples R China 7.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dianbao,Wang, Jing,Wang, Zhe,et al. Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues[J]. SCIENCE OF ADVANCED MATERIALS,2015,7(6):1058-1064.
APA	Zhang, Dianbao.,Wang, Jing.,Wang, Zhe.,Wang, Rui.,Song, Lina.,...&Pang, Xining.(2015).Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues.SCIENCE OF ADVANCED MATERIALS,7(6),1058-1064.
MLA	Zhang, Dianbao,et al."Polyethyleneimine-Coated Fe3O4 Nanoparticles for Efficient siRNA Delivery to Human Mesenchymal Stem Cells Derived from Different Tissues".SCIENCE OF ADVANCED MATERIALS 7.6(2015):1058-1064.