QIBEBT-IR  > 生物传感技术团队(过去)
Biofuel Cell Based Self-Powered Sensing Platform for L-Cysteine Detection
Hou, Chuantao1,2; Fan, Shuqin1,2,3; Lang, Qiaolin1,2; Liu, Aihua1,2,3
2015-03-17
发表期刊ANALYTICAL CHEMISTRY
卷号87期号:6页码:3382-3387
摘要

l-cysteine (l-Cys) detection is of great importance because of its crucial roles in physiological and clinical diagnoses. In this study, a glucose/O-2 biofuel cell (BFC) was assembled by using flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH)-based bioanode and laccase-based biocathode. Interestingly, the open circuit potential (OCP) of the BFC could be inhibited by Cu2+ and subsequently activated by l-Cys, by which a BFC-based self-powered sensing platform for the detection of l-Cys was proposed. The FAD-GDH activity can be inhibited by Cu2+ and, in turn, subsequent reversible activation by l-Cys because of the binding preference of l-Cys toward Cu2+ by forming the CuS bond. The preferential interaction between l-Cys and Cu2+ facilitated Cu2+ to remove from the surface of the bioanode, and thus, the OCP of the system could be turned on. Under optimized conditions, the OCP of the BFC was systematically increased upon the addition of the l-Cys. The OCP increment (OCP) was linear with the concentration of l-Cys within 20 nM to 3 mu M. The proposed sensor exhibited lower detection limit of 10 nM l-Cys (S/N = 3), which is significantly lower than those values for other methods reported so far. Other amino acids and glutathione did not affect l-Cys detection. Therefore, this developed approach is sensitive, facile, cost-effective, and environmental-friendly, and could be very promising for the reliable clinically detecting of l-Cys. This work would trigger the interest of developing BFCs based self-powered sensors for practical applications.

文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1021/ac504694z
关键词[WOS]DEPENDENT GLUCOSE-DEHYDROGENASE ; FUEL-CELLS ; ELECTRODE ; OXIDASE ; SENSOR ; IONS ; PERFORMANCE ; INHIBITION ; BIOSENSORS ; COMPLEX
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000351325800039
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6410
专题生物传感技术团队(过去)
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Hou, Chuantao,Fan, Shuqin,Lang, Qiaolin,et al. Biofuel Cell Based Self-Powered Sensing Platform for L-Cysteine Detection[J]. ANALYTICAL CHEMISTRY,2015,87(6):3382-3387.
APA Hou, Chuantao,Fan, Shuqin,Lang, Qiaolin,&Liu, Aihua.(2015).Biofuel Cell Based Self-Powered Sensing Platform for L-Cysteine Detection.ANALYTICAL CHEMISTRY,87(6),3382-3387.
MLA Hou, Chuantao,et al."Biofuel Cell Based Self-Powered Sensing Platform for L-Cysteine Detection".ANALYTICAL CHEMISTRY 87.6(2015):3382-3387.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Biofuel Cell Based S(1034KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hou, Chuantao]的文章
[Fan, Shuqin]的文章
[Lang, Qiaolin]的文章
百度学术
百度学术中相似的文章
[Hou, Chuantao]的文章
[Fan, Shuqin]的文章
[Lang, Qiaolin]的文章
必应学术
必应学术中相似的文章
[Hou, Chuantao]的文章
[Fan, Shuqin]的文章
[Lang, Qiaolin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Biofuel Cell Based Self-Powered Sensing Platform for L-Cysteine Detection.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。