Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria

doi:10.1007/s00253-014-6273-7

QIBEBT-IR > 微生物代谢工程研究组

	Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria
	Tan, Xiaoming; Du, Wei; Lu, Xuefeng
	2015-03-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	99 期号:5 页码:2147-2154
摘要	Glucosylglycerol (GG) has a range of potential applications in health, pharmacy, and cosmetics due to its physiological, protein-stabilizing, and antioxidative properties. In addition to chemical synthesis and enzymatic catalysis, GG can be produced as a protective osmolyte in salt-stressed bacteria, such as the cyanobacterium Synechocystis sp. PCC 6803. Here, we presented an efficient GG production and secretion by genetically modified and encapsulated Synechocystis cells grown in a semicontinuous manner. We improved the production and secretion of GG in Synechocystis by first disrupting both the ggtC and ggtD genes, which encode the subunits of a GG uptake transporter, as well as the ggpR gene, which encodes a repressor for GG synthesis. Then, we confirmed that the rapid GG release from salt-stressed cells of Synechocystis depended on the ion gradient across the cell membrane. Finally, we proved the feasibility of an agar gel encapsulation method in supporting cell growth and the GG production of Synechocystis under semicontinuous culturing conditions.
文章类型	Article
关键词	Glucosylglycerol Cyanobacteria Metabolic Engineering Gel Encapsulation Salt Shock Hypoosmotic Shock
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-014-6273-7
关键词[WOS]	STRAIN PCC 6803 ; PHOSPHATE SYNTHASE ; OSMOLYTE SYNTHESIS ; GENE ; GGPS ; BIOSYNTHESIS ; EXPRESSION ; PCC-6803
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000350029000010
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6395
专题	微生物代谢工程研究组
作者单位	Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Tan, Xiaoming,Du, Wei,Lu, Xuefeng. Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(5):2147-2154.
APA	Tan, Xiaoming,Du, Wei,&Lu, Xuefeng.(2015).Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(5),2147-2154.
MLA	Tan, Xiaoming,et al."Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.5(2015):2147-2154.