Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)

doi:10.1007/s00253-012-4108-y

QIBEBT-IR > 分子微生物工程研究组

	Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)
	Yuan, Bo 1,3; Hu, Nan 1,2; Sun, Juan 2; Wang, Shi-An1 ; Li, Fu-Li1
	2012-12-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	96 期号:6 页码:1517-1526
摘要	A novel extracellular exoinulinase was purified and characterized from a new yeast strain KRF1(T), and the gene encoding the enzyme was successfully cloned. The enzyme was stable at low pH between 3.0 and 6.5. The K (m) and V (max) values of the purified enzyme for inulin were 2.3 mg/mL and 4.8 mg/min, respectively. The optimum temperature of the inulinase was 50 A degrees C, and the enzyme remained 78 % of activity at 60 A degrees C for 2 h. The inulinase showed an amino acid sequence identity of 58 % to its closest homolog in Meyerozyma (Pichia) guilliermondii. In the secondary structure, the domain G (VMEVH) of the enzyme contained three unique residues (V, M, and H). Compared with previously reported inulinases, the enzyme from strain KRF1(T) displayed strong acid resistance, notable thermostability, and high affinity for the substrate of inulin. Based on sequence analysis of the 26S rDNA D1/D2 domain and phenotypic characterization, the yeast strain KRF1(T) was found to represent a novel anamorphic, ascomycetous yeast species. A complete description of the species is given and the name Candida kutaonensis sp. nov (type strain = KRF1(T) = AS 2.4027(T) = CBS 11388(T)) is proposed.
文章类型	Article
关键词	Candida Kutaonensis Sp Nov. Conserved Motif Inulinase Purification Yeast
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-012-4108-y
关键词[WOS]	CRYPTOCOCCUS-AUREUS G7A ; KLUYVEROMYCES-MARXIANUS ; JERUSALEM-ARTICHOKE ; ASPERGILLUS-NIGER ; PICHIA-GUILLIERMONDII ; MICROBIAL INULINASES ; ETHANOL FERMENTATION ; PURIFIED INULINASE ; SEQUENCE-ANALYSIS ; ENDO-INULINASE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000311244800012
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6390
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Bo,Hu, Nan,Sun, Juan,et al. Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,96(6):1517-1526.
APA	Yuan, Bo,Hu, Nan,Sun, Juan,Wang, Shi-An,&Li, Fu-Li.(2012).Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T).APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,96(6),1517-1526.
MLA	Yuan, Bo,et al."Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 96.6(2012):1517-1526.