| Function of the anal sacs and mid-gut in mitochondrial sulphide metabolism in the echiuran worm Urechis unicinctus | |
Ma, Yu-Bin1,2 ; Zhang, Zhi-Feng1; Shao, Ming-Yu1; Kang, Kyoung-Ho3; Zhang, Li-Tao1; Shi, Xiao-Li1; Dong, Ying-Ping1
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| 2012 | |
| 发表期刊 | MARINE BIOLOGY RESEARCH
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| 卷号 | 8期号:10页码:1026-1031 |
| 摘要 | Sulphides are naturally occurring and widely distributed, poisonous substances and sulphide: quinone oxidoreductase (SQR) has been identified to be responsible for the initial oxidation of sulphide in mitochondria. In a previous study, we found that in the sulphide-adapted species Urechis unicinctus, SQR mRNA concentrations in the mid-gut and in the anal sacs were higher than in the body wall and in the hindgut. To investigate the function of the mid-gut and anal sacs and mitochondrial sulphide metabolism in U. unicinctus, we determined the SQR protein expression in different tissues and the SQR protein expression and enzyme activity after sulphide exposure (25, 50 and 150 mu M) in the anal sacs and in the midgut. The results showed the highest SQR expression was in the anal sacs, followed by the body wall and the hindgut, and finally the mid-gut. During exposure to 50 mu M sulphide, the SQR expression in the anal sacs was significantly increased up to 2 h, reaching a maximum at 24 h and then decreasing up to 48 h. In the anal sacs, SQR enzyme activity was increased significantly up to 6 h and continued to 48 h during exposure to 50 mu M sulphide, whereas in mid-gut, the SQR expression and enzyme activity did not increase significantly. We conclude that the anal sacs act as an important organ while the midgut only acts as an 'assistant' organ for mitochondrial sulphide metabolism in U. unicinctus. |
| 文章类型 | Article |
| 关键词 | Sulphide Adaptation Sulphide:Quinone Oxidoreductase (Sqr) Anal Sacs Mid-gut Urechis Unicinctus |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1080/17451000.2012.707320 |
| 关键词[WOS] | TOLERANT MARINE INVERTEBRATE ; HYDROGEN-SULFIDE ; EXPOSURE ; ADAPTATIONS ; OXIDATION ; TOXICITY ; BINDING ; OXYGEN ; CAUPO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| WOS类目 | Ecology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000309611300010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6337 |
| 专题 | 资源植物与环境工程研究组 |
| 作者单位 | 1.Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 3.Chonnam Natl Univ, Dept Aquaculture, Yeosu, South Korea |
| 推荐引用方式 GB/T 7714 | Ma, Yu-Bin,Zhang, Zhi-Feng,Shao, Ming-Yu,et al. Function of the anal sacs and mid-gut in mitochondrial sulphide metabolism in the echiuran worm Urechis unicinctus[J]. MARINE BIOLOGY RESEARCH,2012,8(10):1026-1031. |
| APA | Ma, Yu-Bin.,Zhang, Zhi-Feng.,Shao, Ming-Yu.,Kang, Kyoung-Ho.,Zhang, Li-Tao.,...&Dong, Ying-Ping.(2012).Function of the anal sacs and mid-gut in mitochondrial sulphide metabolism in the echiuran worm Urechis unicinctus.MARINE BIOLOGY RESEARCH,8(10),1026-1031. |
| MLA | Ma, Yu-Bin,et al."Function of the anal sacs and mid-gut in mitochondrial sulphide metabolism in the echiuran worm Urechis unicinctus".MARINE BIOLOGY RESEARCH 8.10(2012):1026-1031. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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