| Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall | |
| Scholz, Matthew J.1; Weiss, Taylor L.2; Jinkerson, Robert E.1; Jing, Jia3,4; Roth, Robyn5; Goodenough, Ursula2; Posewitz, Matthew C.1; Gerken, Henri G.6 | |
| 2014-11-01 | |
| 发表期刊 | EUKARYOTIC CELL
 |
| 卷号 | 13期号:11页码:1450-1464 |
| 摘要 | Marine algae of the genus Nannochloropsis are promising producers of biofuel precursors and nutraceuticals and are also harvested commercially for aquaculture feed. We have used quick-freeze, deep-etch electron microscopy, Fourier transform infrared spectroscopy, and carbohydrate analyses to characterize the architecture of the Nannochloropsis gaditana (strain CCMP 526) cell wall, whose recalcitrance presents a significant barrier to biocommodity extraction. The data indicate a bilayer structure consisting of a cellulosic inner wall (similar to 75% of the mass balance) protected by an outer hydrophobic algaenan layer. Cellulase treatment of walls purified after cell lysis generates highly enriched algaenan preparations without using the harsh chemical treatments typically used in algaenan isolation and characterization. Nannochloropsis algaenan was determined to comprise long, straight-chain, saturated aliphatics with ether cross-links, which closely resembles the cutan of vascular plants. Chemical identification of >85% of the isolated cell wall mass is detailed, and genome analysis is used to identify candidate biosynthetic enzymes. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1128/EC.00183-14 |
| 关键词[WOS] | HAEMATOCOCCUS-PLUVIALIS CHLOROPHYCEAE ; CHLAMYDOMONAS-MONOICA CHLOROPHYTA ; PLANT CELLULOSE SYNTHASE ; FRESH-WATER ALGAE ; BRAUNII RACE B ; SCENEDESMUS CHLOROPHYCEAE ; BOTRYOCOCCUS-BRAUNII ; EXTRACELLULAR-MATRIX ; TRICHODERMA-REESEI ; MARINE MICROALGAE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000344646600011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6318 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA 2.Washington Univ, Dept Biol, St Louis, MO 63130 USA 3.Chinese Acad Sci, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, BioEnergy Genome Ctr, Qindao Inst BioEnergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 5.Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA 6.Arizona State Univ, Arizona Ctr Algal Technol & Innovat, Mesa, AZ 85212 USA |
| 推荐引用方式 GB/T 7714 | Scholz, Matthew J.,Weiss, Taylor L.,Jinkerson, Robert E.,et al. Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall[J]. EUKARYOTIC CELL,2014,13(11):1450-1464. |
| APA | Scholz, Matthew J..,Weiss, Taylor L..,Jinkerson, Robert E..,Jing, Jia.,Roth, Robyn.,...&Gerken, Henri G..(2014).Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall.EUKARYOTIC CELL,13(11),1450-1464. |
| MLA | Scholz, Matthew J.,et al."Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall".EUKARYOTIC CELL 13.11(2014):1450-1464. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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