| QSpec: online control and data analysis systemfor single-cell Raman spectroscopy | |
Ren, Lihui; Su, Xiaoquan; Wang, Yun; Xu, Jian ; Ning, Kang1
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| 2014-06-26 | |
| 发表期刊 | PEERJ
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| 卷号 | 2 |
| 摘要 | Single-cell phenotyping is critical to the success of biological reductionism. Raman-activated cell sorting (RACS) has shown promise in resolving the dynamics of living cells at the individual level and to uncover population heterogeneities in comparison to established approaches such as fluorescence-activated cell sorting (FACS). Given that the number of single-cells would be massive in any experiment, the power of Raman profiling technique for single-cell analysis would be fully utilized only when coupled with a high-throughput and intelligent process control and data analysis system. In this work, we established QSpec, an automatic system that supports high-throughput Raman-based single-cell phenotyping. Additionally, a single-cell Raman profile database has been established upon which data-mining could be applied to discover the heterogeneity among single-cells under different conditions. To test the effectiveness of this control and data analysis system, a sub-system was also developed to simulate the phenotypes of single-cells as well as the device features. |
| 文章类型 | Article |
| 关键词 | Single-cell High-throughput Raman-activated Cell Sorting (Racs) Data Analysis Database Simulation |
| WOS标题词 | Science & Technology |
| DOI | 10.7717/peerj.436 |
| 关键词[WOS] | MICROBIAL-CELLS ; TWEEZERS ; ARRAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000347611300002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6308 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Chinese Acad Sci, Single Cell Ctr, Bioinformat Grp, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Lihui,Su, Xiaoquan,Wang, Yun,et al. QSpec: online control and data analysis systemfor single-cell Raman spectroscopy[J]. PEERJ,2014,2. |
| APA | Ren, Lihui,Su, Xiaoquan,Wang, Yun,Xu, Jian,&Ning, Kang.(2014).QSpec: online control and data analysis systemfor single-cell Raman spectroscopy.PEERJ,2. |
| MLA | Ren, Lihui,et al."QSpec: online control and data analysis systemfor single-cell Raman spectroscopy".PEERJ 2(2014). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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