FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis

doi:10.1371/journal.pone.0099741

QIBEBT-IR > 资源植物与环境工程研究组

	FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis
	Lu, Xiaoduo 1; Zhang, Dongyuan 2; Li, Shipeng 1; Su, Yanping 3; Liang, Qiuju 4,5; Meng, Hongyan 4; Shen, Songdong 3; Fan, Yunliu 4,5; Liu, Chunming 6; Zhang, Chunyi 4,5
	2014-06-25
发表期刊	PLOS ONE
卷号	9 期号:6
摘要	Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are present in the Arabidopsis genome. In this study, we showed that T-DNA insertion mutations in the Arabidopsis FtsHi4 gene resulted in embryo arrest at the globular-to-heart-shaped transition stage. Transmission electron microscopic analyses revealed abnormal plastid differentiation with a severe defect in thylakoid formation in the mutant embryos. Immunocytological studies demonstrated that FtsHi4 localized in chloroplasts as a thylakoid membrane-associated protein, supporting its essential role in thylakoid membrane formation. We further showed that FtsHi4 forms protein complexes, and that there was a significant reduction in the accumulation of D2 and PsbO (two photosystem II proteins) in mutant ovules. The role of FtsHi4 in chloroplast development was confirmed using an RNA-interfering approach. Additionally, mutations in other FtsHi genes including FtsHi1, FtsHi2, and FtsHi5 caused phenotypic abnormalities similar to ftshi4 with respect to plastid differentiation during embryogenesis. Taken together, our data suggest that FtsHi4, together with FtsHi1, FtsHi2, and FtsHi5 are essential for chloroplast development in Arabidopsis.
文章类型	Article
WOS标题词	Science & Technology
DOI	10.1371/journal.pone.0099741
关键词[WOS]	PHOTOSYSTEM-II REPAIR ; THYLAKOID MEMBRANE ; EMBRYO DEVELOPMENT ; GENE-EXPRESSION ; CHLOROPHYLL FLUORESCENCE ; PLANT DEVELOPMENT ; LEAF VARIEGATION ; SEED DEVELOPMENT ; PROTEIN IMPORT ; D1 PROTEIN
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000338709500017
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6288
专题	资源植物与环境工程研究组
作者单位	1.Qilu Normal Univ, Dept Life Sci, Jinan, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 3.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou, Peoples R China 4.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China 5.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xiaoduo,Zhang, Dongyuan,Li, Shipeng,et al. FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis[J]. PLOS ONE,2014,9(6).
APA	Lu, Xiaoduo.,Zhang, Dongyuan.,Li, Shipeng.,Su, Yanping.,Liang, Qiuju.,...&Zhang, Chunyi.(2014).FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis.PLOS ONE,9(6).
MLA	Lu, Xiaoduo,et al."FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis".PLOS ONE 9.6(2014).