Acetobacteroides hydrogenigenes gen. nov., sp nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp | |
Su, Xiao-Li1,2; Tian, Qi1,3; Zhang, Jie1,2; Yuan, Xian-Zheng1; Shi, Xiao-Shuang1; Guo, Rong-Bo1; Qiu, Yan-Ling1 | |
2014-09-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
卷号 | 64期号:1页码:2986-2991 |
摘要 | A strictly anaerobic, mesophilic, carbohydrate-fermenting, hydrogen-producing bacterium, designated strain RL-C-T, was isolated from a reed swamp in China. Cells were Gram-stain-negative, catalase-negative, non-spore-forming, non-motile rods measuring 0.7-1.0 mu m in width and 3.0-8.0 mu m in length. The optimum temperature for growth of strain RL-C-T was 37 degrees C (range 25-40 degrees C) and pH 7.0-7.5 (range pH 5.7-8.0). The strain could grow fermentatively on yeast extract, tryptone, arabinose, glucose, galactose, mannose, maltose, lactose, glycogen, pectin and starch. The main end products of glucose fermentation were acetate, H-2 and CO2. Organic acids, alcohols and amino acids were not utilized for growth. Yeast extract was not required for growth; however, it stimulated growth slightly. Nitrate, sulfate, sulfite, thiosulfate, elemental sulfur and Fe(III) nitrilotriacetate were not reduced as terminal electron acceptors. Aesculin was hydrolysed but not gelatin. Indole and H2S were produced from yeast extract. The G+C content of the genomic DNA was 51.2 mol%. The major cellular fatty acids were iso-C-15:0, anteiso-C-(15:0) and C-16:0. The most abundant polar lipid of strain RL-C-T was phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that the isolate belongs to the uncultured Blvii28 wastewater-sludge group (http://www.arb-silva.de/) in the family Rikenellaceae of the phylum Bacteroidetes, and shared low sequence similarities with the related species Alistipes shahii WAL 8301(T) (81.8 %), Rikenella microfusus ATCC 29728(T) (81.7%) and Anaerocella delicata WN081(T) (80.9 %). On the basis of these data, a novel species in a new genus of the family Rikenellaceae is proposed, Acetobacteroides hydrogenigenes gen. nov., sp. nov. The type strain of the type species is RL-C-T (=JCM 17603(T)=DSM 24657(T)=CGMCC 1.5173(T)). |
文章类型 | Article |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1099/ijs.0.063917-0 |
关键词[WOS] | DIVERSITY ; MEMBERS ; SLUDGE |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
WOS记录号 | WOS:000344912300010 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6284 |
专题 | 工业生物燃气研究组 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266101, Peoples R China |
推荐引用方式 GB/T 7714 | Su, Xiao-Li,Tian, Qi,Zhang, Jie,et al. Acetobacteroides hydrogenigenes gen. nov., sp nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2014,64(1):2986-2991. |
APA | Su, Xiao-Li.,Tian, Qi.,Zhang, Jie.,Yuan, Xian-Zheng.,Shi, Xiao-Shuang.,...&Qiu, Yan-Ling.(2014).Acetobacteroides hydrogenigenes gen. nov., sp nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,64(1),2986-2991. |
MLA | Su, Xiao-Li,et al."Acetobacteroides hydrogenigenes gen. nov., sp nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.1(2014):2986-2991. |
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