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Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan
Cheng, Xinwei1,3; Su, Xiaoquan2; Chen, Xiaohua2; Zhao, Huanxin1; Bo, Cunpei2; Xu, Jian2; Bai, Hong1; Ning, Kang2
2014-06-03
Source PublicationSCIENTIFIC REPORTS
Volume4Issue:1Pages:1
Abstract

Although Traditional Chinese Medicine (TCM) preparations have long history with successful applications, the scientific and systematic quality assessment of TCM preparations mainly focuses on chemical constituents and is far from comprehensive. There are currently only few primitive studies on assessment of biological ingredients in TCM preparations. Here, we have proposed a method, M-TCM, for biological assessment of the quality of TCM preparations based on high-throughput sequencing and metagenomic analysis. We have tested this method on Liuwei Dihuang Wan (LDW), a TCM whose ingredients have been well-defined. Our results have shown that firstly, this method could determine the biological ingredients of LDW preparations. Secondly, the quality and stability of LDW varies significantly among different manufacturers. Thirdly, the overall quality of LDW samples is significantly affected by their biological contaminations. This novel strategy has the potential to achieve comprehensive ingredient profiling of TCM preparations.

SubtypeArticle
WOS HeadingsScience & Technology
DOI10.1038/srep05147
WOS KeywordQUALITY-CONTROL ; HERBAL DRUGS ; DNA BARCODES ; IDENTIFICATION ; PLANTS ; AUTHENTICATION ; PRODUCTS ; SYSTEM ; FAMILY ; ITS2
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000336655300005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6282
Collection单细胞中心组群
Affiliation1.Shandong Acad Med Sci, Inst Mat Med, Jinan 250062, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels & Bioinformat, Grp SingleCell Ctr,Shandong Key Lab Energy Genet, Qingdao 266101, Peoples R China
3.Univ Jinan, Shandong Acad Med Sci, Sch Med & Life Sci, Jinan 250062, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Xinwei,Su, Xiaoquan,Chen, Xiaohua,et al. Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan[J]. SCIENTIFIC REPORTS,2014,4(1):1.
APA Cheng, Xinwei.,Su, Xiaoquan.,Chen, Xiaohua.,Zhao, Huanxin.,Bo, Cunpei.,...&Ning, Kang.(2014).Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan.SCIENTIFIC REPORTS,4(1),1.
MLA Cheng, Xinwei,et al."Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan".SCIENTIFIC REPORTS 4.1(2014):1.
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