Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition

doi:10.1111/jipb.12275

QIBEBT-IR > 微生物代谢工程研究组

	Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition
	Yang, Haomeng 1; Liao, Libing 1,2; Bo, Tingting 1,2; Zhao, Lei 1,2; Sun, Xuwu 1; Lu, Xuefeng 4; Norling, Birgitta 3; Huang, Fang 1
	2014-12-01
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
卷号	56 期号:12 页码:1136-1150
摘要	Cyanobacteria are ancient photosynthetic prokaryotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem II (PSII) in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited. We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp. PCC 6803. Here, we report that Slr0151 is involved in PSII repair of the organism. We generated a mutant strain (Dslr0151) lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSII activity in Synechocystis. Moreover, the mutant exhibited increased photoinhibition due to impairment of PSII repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSII repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSII in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together, these results indicate that Slr0151 plays an important role in regulating PSII repair in the organism under high-light stress condition.
文章类型	Article
关键词	Cyanobacteria Membrane Protein Photosystem Ii Repair Slr0151 Synechocystis
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1111/jipb.12275
关键词[WOS]	SP STRAIN PCC-6803 ; ENVIRONMENTAL-STRESS ; PLASMA-MEMBRANES ; GENE-EXPRESSION ; D1 PROTEIN ; COMPLEX ; CYANOBACTERIA ; PHOTOINHIBITION ; ACCUMULATION ; PROTEOMICS
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000345845200004
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6185
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Yang, Haomeng,Liao, Libing,Bo, Tingting,et al. Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2014,56(12):1136-1150.
APA	Yang, Haomeng.,Liao, Libing.,Bo, Tingting.,Zhao, Lei.,Sun, Xuwu.,...&Huang, Fang.(2014).Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,56(12),1136-1150.
MLA	Yang, Haomeng,et al."Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 56.12(2014):1136-1150.