pH-Switchable Electrochemical Sensing Platform based on Chitosan-Reduced Graphene Oxide/Concanavalin A Layer for Assay of Glucose and Urea

doi:10.1021/ac402742m

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	pH-Switchable Electrochemical Sensing Platform based on Chitosan-Reduced Graphene Oxide/Concanavalin A Layer for Assay of Glucose and Urea
	Song, Yonghai 1; Liu, Hongyu 1; Tan, Hongliang 1; Xu, Fugang 1; Jia, Jianbo 2; Zhang, Lixue3 ; Li, Zhuang 2; Wang, Li 1
	2014-02-18
发表期刊	ANALYTICAL CHEMISTRY
卷号	86 期号:4 页码:1980-1987
摘要	A facile and effective electrochemical sensing platform for the detection of glucose and urea in one sample without separation was developed using chitosan-reduced graphene oxide (CS-rGO)/concanavalin A (Con A) as a sensing layer. The CS-rGO/Con A with pH-dependent surface net charges exhibited pH-switchable response to negatively charged Fe(CN)(6)(3-). The principle for glucose and urea detection was essentially based on in situ pH-switchable enzyme-catalyzed reaction in which the oxidation of glucose catalyzed by glucose oxidase or the hydrolyzation of urea catalyzed by urease resulted in a pH change of electrolyte solution to give different electrochemical responses toward Fe(CN)(6)(3-). It was verified by cyclic voltammograms, differential pulse voltammograms, and electrochemical impedance spectroscopy. The resistance to charge transfer or amperometric current changed proportionally toward glucose concentration from 1.0 to 10.0 mM and urea concentration from 1.0 to 7.0 mM. On the basis of human serum experiments, the sensing platform was proved to be suitable for simultaneous assay of glucose and urea in a practical biosystem. This work not only gives a way to detect glucose and urea in one sample without separation but also provides a potential strategy for the detection of nonelectroactive species based on the enzyme-catalyzed reaction and pH-switchable biosensor.
文章类型	Article
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1021/ac402742m
关键词[WOS]	PROTEIN BIO-INTERPHASE ; AG NANOPARTICLES ; CONCANAVALIN-A ; ELECTRON-TRANSFER ; HYDROGEN-PEROXIDE ; CYTOCHROME-C ; IN-SITU ; BIOSENSOR ; FILMS ; OXIDE
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000331775600010
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6168
专题	仿生与固态能源系统研究组
作者单位	1.Jiangxi Normal Univ, Coll Chem & Chem Engn, Nanchang 330022, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Song, Yonghai,Liu, Hongyu,Tan, Hongliang,et al. pH-Switchable Electrochemical Sensing Platform based on Chitosan-Reduced Graphene Oxide/Concanavalin A Layer for Assay of Glucose and Urea[J]. ANALYTICAL CHEMISTRY,2014,86(4):1980-1987.
APA	Song, Yonghai.,Liu, Hongyu.,Tan, Hongliang.,Xu, Fugang.,Jia, Jianbo.,...&Wang, Li.(2014).pH-Switchable Electrochemical Sensing Platform based on Chitosan-Reduced Graphene Oxide/Concanavalin A Layer for Assay of Glucose and Urea.ANALYTICAL CHEMISTRY,86(4),1980-1987.
MLA	Song, Yonghai,et al."pH-Switchable Electrochemical Sensing Platform based on Chitosan-Reduced Graphene Oxide/Concanavalin A Layer for Assay of Glucose and Urea".ANALYTICAL CHEMISTRY 86.4(2014):1980-1987.