Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold

doi:10.1016/j.bbrc.2014.05.077

QIBEBT-IR > 代谢物组学研究组

	Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold
	Feng, Yingang1 ; Song, Xiaxia 2; Lin, Jinzhong 3; Xuan, Jinsong 2; Cui, Qiu1 ; Wang, Jinfeng 3
	2014-07-18
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷号	450 期号:1 页码:67-72
摘要	Three archaea-specific ribosomal proteins recently identified show no sequence homology with other known proteins. Here we determined the structure of L46a, the most conserved one among the three proteins, from Sulfolobus solfataricus P2 using NMR spectroscopy. The structure presents a twisted beta-sheet formed by the N-terminal part and two helices at the C-terminus. The L46a structure has a positively charged surface which is conserved in the L46a protein family and is the potential rRNA-binding site. Searching homologous structures in Protein Data Bank revealed that the structure of L46a represents a novel protein fold. The backbone dynamics identified by NMR relaxation experiments reveal significant flexibility at the rRNA binding surface. The potential position of L46a on the ribosome was proposed by fitting the structure into a previous electron microscopy map of the ribosomal 50S subunit, which indicated that L46a contacts to domain I of 23S rRNA near a multifunctional ribosomal protein L7ae. (C) 2014 Elsevier Inc. All rights reserved.
文章类型	Article
关键词	Ribosomal Protein Protein Structure Archaea Nmr Protein Fold
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.bbrc.2014.05.077
关键词[WOS]	BACKBONE DYNAMICS ; NOE ASSIGNMENT ; NMR ; RELAXATION ; DATABASE ; PROGRAM ; SYSTEM
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000343641000012
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6129
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Dept Biol Sci & Engn, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Feng, Yingang,Song, Xiaxia,Lin, Jinzhong,et al. Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,450(1):67-72.
APA	Feng, Yingang,Song, Xiaxia,Lin, Jinzhong,Xuan, Jinsong,Cui, Qiu,&Wang, Jinfeng.(2014).Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,450(1),67-72.
MLA	Feng, Yingang,et al."Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 450.1(2014):67-72.