Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose

doi:10.1128/AEM.00295-14

QIBEBT-IR > 分子微生物工程研究组

	Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose
	Zhang, Kundi 1,3; Chen, Xiaohua 1; Schwarz, Wolfgang H.2; Li, Fuli 1
	2014-04-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号	80 期号:8 页码:2592-2601
摘要	Two cellulolytic thermophilic bacterial strains, CS-3-2 and CS-4-4, were isolated from decayed cornstalk by the addition of growth-supporting factors to the medium. According to 16S rRNA gene-sequencing results, these strains belonged to the genus Clostridium and showed 98.87% and 98.86% identity with Clostridium stercorarium subsp. leptospartum ATCC 35414(T) and Clostridium cellulosi AS 1.1777(T), respectively. The endoglucanase and exoglucanase activities of strain CS-4-4 were approximately 3 to 5 times those of strain CS-3-2, whereas the beta-glucosidase activity of strain CS-3-2 was 18 times higher than that of strain CS-4-4. The xylanase activity of strain CS-3-2 was 9 times that of strain CS-4-4, whereas the beta-xylosidase activity of strain CS-4-4 was 27 times that of strain CS-3-2. The enzyme activities in spent cultures following cocultivation of the two strains with cornstalk as the substrate were much greater than those in pure cultures or an artificial mixture of samples, indicating synergism of glycoside hydrolase secretomes between the two strains. Quantitative measurement of the two strains in the cocultivation system indicated that strain CS-3-2 grew robustly during the initial stages, whereas strain CS-4-4 dominated the system in the late-exponential phase. Liquid chromatography-tandem mass spectrometry analysis of protein bands appearing in the native zymograms showed that ORF3880 and ORF3883 from strain CS-4-4 played key roles in the lignocellulose degradation process. Both these open reading frames (ORFs) exhibited endoglucanase and xylanase activities, but ORF3880 showed tighter adhesion to insoluble substrates at 4, 25, and 60 degrees C owing to its five carbohydrate-binding modules (CBMs).
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1128/AEM.00295-14
关键词[WOS]	CARBOHYDRATE-BINDING MODULES ; GROWTH-SUPPORTING FACTORS ; CLOSTRIDIUM-THERMOCELLUM ; GEOBACILLUS-TOEBII ; SP NOV. ; PROCESSIVE ENDOGLUCANASE ; ANAEROBIC THERMOPHILES ; SYMBIOBACTERIUM-TOEBII ; CELLULOSE HYDROLYSIS ; ENZYMATIC-HYDROLYSIS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000333531600029
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6126
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 2.Tech Univ Munich, Lehrstuhl Mikrobiol, Freising Weihenstephan, Germany 3.Univ Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Kundi,Chen, Xiaohua,Schwarz, Wolfgang H.,et al. Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(8):2592-2601.
APA	Zhang, Kundi,Chen, Xiaohua,Schwarz, Wolfgang H.,&Li, Fuli.(2014).Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(8),2592-2601.
MLA	Zhang, Kundi,et al."Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.8(2014):2592-2601.