Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae

doi:10.1105/tpc.113.121418

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	Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
	Li, Jing1,2,3 ; Han, Danxiang 4; Wang, Dongmei1,2 ; Ning, Kang 1,2; Jia, Jing 1,2,3; Wei, Li 1,2,3; Jing, Xiaoyan1,2 ; Huang, Shi 1,2,3; Chen, Jie 1,2; Li, Yantao 5,6; Hu, Qiang 7; Xu, Jian1,2
	2014-04-01
发表期刊	PLANT CELL
卷号	26 期号:4 页码:1645-1665
摘要	To reveal the molecular mechanisms of oleaginousness in microalgae, transcriptomic and lipidomic dynamics of the oleaginous microalga Nannochloropsis oceanica IMET1 under nitrogen-replete (N+) and N-depleted (N-) conditions were simultaneously tracked. At the transcript level, enhanced triacylglycerol (TAG) synthesis under N-conditions primarily involved upregulation of seven putative diacylglycerol acyltransferase (DGAT) genes and downregulation of six other DGAT genes, with a simultaneous elevation of the other Kennedy pathway genes. Under N-conditions, despite downregulation of most de novo fatty acid synthesis genes, the pathways that shunt carbon precursors from protein and carbohydrate metabolic pathways into glycerolipid synthesis were stimulated at the transcript level. In particular, the genes involved in supplying carbon precursors and energy for de novo fatty acid synthesis, including those encoding components of the pyruvate dehydrogenase complex (PDHC), glycolysis, and PDHC bypass, and suites of specific transporters, were substantially upregulated under N-conditions, resulting in increased overall TAG production. Moreover, genes involved in the citric acid cycle and beta-oxidation in mitochondria were greatly enhanced to utilize the carbon skeletons derived from membrane lipids and proteins to produce additional TAG or its precursors. This temporal and spatial regulation model of oil accumulation in microalgae provides a basis for improving our understanding of TAG synthesis in microalgae and will also enable more rational genetic engineering of TAG production.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1105/tpc.113.121418
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; FATTY-ACID SYNTHESIS ; DIATOM CYCLOTELLA-CRYPTICA ; ACYL CARRIER PROTEIN ; CHLAMYDOMONAS-REINHARDTII ; TRIACYLGLYCEROL ACCUMULATION ; NITROGEN STARVATION ; ELECTROSPRAY-IONIZATION ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS记录号	WOS:000337351300021
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6112
专题	单细胞中心组群
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Arizona State Univ, Dept Appl Biol Sci, Lab Algae Res & Biotechnol, Mesa, AZ 85212 USA 5.Univ Maryland, Ctr Environm, Inst Marine & Environm Technol, Baltimore, MD 21202 USA 6.Univ Maryland Baltimore Cty, Baltimore, MD 21202 USA 7.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Han, Danxiang,Wang, Dongmei,et al. Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae[J]. PLANT CELL,2014,26(4):1645-1665.
APA	Li, Jing.,Han, Danxiang.,Wang, Dongmei.,Ning, Kang.,Jia, Jing.,...&Xu, Jian.(2014).Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae.PLANT CELL,26(4),1645-1665.
MLA	Li, Jing,et al."Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae".PLANT CELL 26.4(2014):1645-1665.