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Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community
Zhang, Dayi1; Berry, James P.1; Zhu, Di1; Wang, Yun2,3; Chen, Yin4; Jiang, Bo5; Huang, Shi2,3; Langford, Harry1; Li, Guanghe5; Davison, Paul A.1; Xu, Jian2,3; Aries, Eric6; Huang, Wei E.1
2015-03-01
Source PublicationISME JOURNAL
Volume9Issue:3Pages:603-614
Abstract

Although uncultured microorganisms have important roles in ecosystems, their ecophysiology in situ remains elusive owing to the difficulty of obtaining live cells from their natural habitats. In this study, we employed a novel magnetic nanoparticle-mediated isolation (MMI) method to recover metabolically active cells of a group of previously uncultured phenol degraders, Burkholderiales spp., from coking plant wastewater biosludge; five other culturable phenol degraders-Rhodococcus sp., Chryseobacterium sp. and three different Pseudomonas spp.-were also isolated from the same biosludge using traditional methods. The kinetics of phenol degradation by MMI-recovered cells (MRCs) was similar to that of the original sludge. Stable isotope probing (SIP) and pyrosequencing of the 16S rRNA from the 'heavy' DNA (C-13-DNA) fractions indicated that Burkholderiales spp. were the key phenol degraders in situ in the biosludge, consistent with the results of MRCs. Single-cell Raman micro-spectroscopy was applied to probe individual bacteria in the MRCs obtained from the SIP experiment and showed that 79% of them were fully C-13-labelled. Biolog assays on the MRCs revealed the impact of various carbon and nitrogen substrates on the efficiency of phenol degradation in the wastewater treatment plant biosludge. Specifically, hydroxylamine, a metabolite of ammonia oxidisation, but not nitrite, nitrate or ammonia, inhibited phenol degradation in the biosludge. Our results provided a novel insight into the occasional abrupt failure events that occur in the wastewater treatment plant. This study demonstrated that MMI is a powerful tool to recover live and functional cells in situ from a complex microbial community to enable further characterisation of their physiology.

SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1038/ismej.2014.161
WOS KeywordIN-SITU HYBRIDIZATION ; RAMAN-SPECTROSCOPY ; AMMONIA OXIDATION ; RIBOSOMAL-RNA ; METAGENOMICS ; PHENOL ; MICROORGANISMS ; PHYLOGENY ; MAJORITY ; ARCHAEA
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Microbiology
WOS SubjectEcology ; Microbiology
WOS IDWOS:000349850600007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6086
Collection单细胞中心组群
Affiliation1.Univ Sheffield, Dept Civil & Struct Engn, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Peoples R China
3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Peoples R China
4.Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England
5.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China
6.Tata Steel, Grp Environm, Swinden Technol Ctr, Rotherham, S Yorkshire, England
Recommended Citation
GB/T 7714
Zhang, Dayi,Berry, James P.,Zhu, Di,et al. Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community[J]. ISME JOURNAL,2015,9(3):603-614.
APA Zhang, Dayi.,Berry, James P..,Zhu, Di.,Wang, Yun.,Chen, Yin.,...&Huang, Wei E..(2015).Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community.ISME JOURNAL,9(3),603-614.
MLA Zhang, Dayi,et al."Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community".ISME JOURNAL 9.3(2015):603-614.
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