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Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release
Zhang, Peiran1,2,3; Ren, Lihui1,2; Zhang, Xu1,2; Shan, Yufei1,2; Wang, Yun1,2; Ji, Yuetong1,2; Yin, Huabing5; Huang, Wei E.1,2,4; Xu, Jian1,2; Ma, Bo1,2
2015-02-17
发表期刊ANALYTICAL CHEMISTRY
卷号87期号:4页码:2282-2289
摘要Raman-activated cell sorting (RACS) is a promising single-cell technology that holds several significant advantages, as RACS is label-free, information-rich, and potentially in situ. To date, the ability of the technique to identify single cells in a high-speed flow has been limited by inherent weakness of the spontaneous Raman signal. Here we present an alternative pause-and-sort RACS microfluidic system that combines positive dielectrophoresis (pDEP) for single-cell trap and release with a solenoid-valve-suction-based switch for cell separation. This has allowed the integration of trapping, Raman identification, and automatic separation of individual cells in a high-speed flow. By exerting a periodical pDEP field, single cells were trapped, ordered, and positioned individually to the detection point for Raman measurement. As a proof-of-concept demonstration, a mixture of two cell strains containing carotenoid-producing yeast (9%) and non-carotenoid-producing Saccharomyces cerevisiae (91%) was sorted, which enriched the former to 73% on average and showed a fast Raman-activated cell sorting at the subsecond level.
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1021/ac503974e
关键词[WOS]MICROBIAL-CELLS ; IDENTIFICATION ; SPECTROSCOPY ; SERS ; MICROSPECTROSCOPY ; MANIPULATION ; TWEEZERS ; MICROORGANISMS ; MICROALGAE ; PATHOGENS
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000349806200038
引用统计
被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6084
专题单细胞中心
作者单位1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
4.Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
5.Univ Glasgow, Sch Engn, Div Biomed Engn, Glasgow G12 8LT, Lanark, Scotland
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Zhang, Peiran,Ren, Lihui,Zhang, Xu,et al. Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release[J]. ANALYTICAL CHEMISTRY,2015,87(4):2282-2289.
APA Zhang, Peiran.,Ren, Lihui.,Zhang, Xu.,Shan, Yufei.,Wang, Yun.,...&Ma, Bo.(2015).Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release.ANALYTICAL CHEMISTRY,87(4),2282-2289.
MLA Zhang, Peiran,et al."Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release".ANALYTICAL CHEMISTRY 87.4(2015):2282-2289.
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