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Title:
Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release
Author: Zhang, Peiran1,2,3; Ren, Lihui1,2; Zhang, Xu1,2; Shan, Yufei1,2; Wang, Yun1,2; Ji, Yuetong1,2; Yin, Huabing5; Huang, Wei E.1,2,4; Xu Jian(徐健)1,2; Ma, Bo1,2
Source: ANALYTICAL CHEMISTRY
Issued Date: 2015-02-17
Volume: 87, Issue:4, Pages:2282-2289
DOI: 10.1021/ac503974e
DOC Type: Article
English Abstract: Raman-activated cell sorting (RACS) is a promising single-cell technology that holds several significant advantages, as RACS is label-free, information-rich, and potentially in situ. To date, the ability of the technique to identify single cells in a high-speed flow has been limited by inherent weakness of the spontaneous Raman signal. Here we present an alternative pause-and-sort RACS microfluidic system that combines positive dielectrophoresis (pDEP) for single-cell trap and release with a solenoid-valve-suction-based switch for cell separation. This has allowed the integration of trapping, Raman identification, and automatic separation of individual cells in a high-speed flow. By exerting a periodical pDEP field, single cells were trapped, ordered, and positioned individually to the detection point for Raman measurement. As a proof-of-concept demonstration, a mixture of two cell strains containing carotenoid-producing yeast (9%) and non-carotenoid-producing Saccharomyces cerevisiae (91%) was sorted, which enriched the former to 73% on average and showed a fast Raman-activated cell sorting at the subsecond level.
WOS Headings: Science & Technology ; Physical Sciences
WOS Subject: Chemistry, Analytical
WOS Subject Extended: Chemistry
WOS Keyword Plus: MICROBIAL-CELLS ; IDENTIFICATION ; SPECTROSCOPY ; SERS ; MICROSPECTROSCOPY ; MANIPULATION ; TWEEZERS ; MICROORGANISMS ; MICROALGAE ; PATHOGENS
Indexed Type: SCI
Language: 英语
WOS ID: WOS:000349806200038
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/6084
Appears in Collections:单细胞中心_期刊论文

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description.institution: 1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
4.Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
5.Univ Glasgow, Sch Engn, Div Biomed Engn, Glasgow G12 8LT, Lanark, Scotland

Recommended Citation:
Zhang, Peiran,Ren, Lihui,Zhang, Xu,et al. Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release[J]. ANALYTICAL CHEMISTRY,2015,87(4):2282-2289.
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