Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid

doi:10.1002/cbic.201402600

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	Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid
	Zhang, Wei 1; Cao, Shaona 1; Qiu, Li 2; Qi, Fengxia1 ; Li, Zhong 1,3; Yang, Ying1 ; Huang, Shaohua 1; Bai, Fali1 ; Liu, Changning 4; Wan, Xiaobo 2; Li, Shengying 1
	2015-03-02
发表期刊	CHEMBIOCHEM
卷号	16 期号:4 页码:565-569
摘要	Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG gene was functionally and kinetically characterized in vitro. MpaG catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis.
文章类型	Article
关键词	Biosynthesis Methyltransferases Mycophenolic Acid Substrate Flexibility Transferases
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/cbic.201402600
关键词[WOS]	PENICILLIUM-BREVICOMPACTUM ; DEHYDROGENASE ; FUNGI
收录类别	SCI ; IC
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Medicinal
WOS记录号	WOS:000350138700005
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6083
专题	酶工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci Menglun, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Cao, Shaona,Qiu, Li,et al. Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid[J]. CHEMBIOCHEM,2015,16(4):565-569.
APA	Zhang, Wei.,Cao, Shaona.,Qiu, Li.,Qi, Fengxia.,Li, Zhong.,...&Li, Shengying.(2015).Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid.CHEMBIOCHEM,16(4),565-569.
MLA	Zhang, Wei,et al."Functional Characterization of MpaG ', the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid".CHEMBIOCHEM 16.4(2015):565-569.