Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry

doi:10.1002/jssc.201300012

QIBEBT-IR > 工业生物燃气研究组

	Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry
	Guo, Na 1; Yang, Dawei 2; Ablajan, Keyume 3; Niu, Xiaohong 1; Fan, Bin 1; Wang, Zhiguo 1; Dai, Jingang 1; Wu, Xiaoxia 1; Liu, Baidong 1
	2013-06-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	36 期号:12 页码:1953-1958
摘要	A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra- and interday precisions (RSD < 5.07%) with good accuracies (97.76-105.08%) and good recovery (95.0-107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30-17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.
文章类型	Article
关键词	Aconitum Alkaloids Baifupian Fuzi Heishunpian Rapid Resolution Lc
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201300012
关键词[WOS]	ACONITUM ALKALOIDS ; DITERPENOID ALKALOIDS ; MEDICINE FUZI ; CHINESE ; PHARMACOKINETICS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000320553800011
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6051
专题	工业生物燃气研究组
作者单位	1.China Acad Chinese Med Sci, Expt Res Ctr, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao, Peoples R China 3.Xinjiang Univ, Coll Chem & Chem Engn, Urumqi, Peoples R China
推荐引用方式 GB/T 7714	Guo, Na,Yang, Dawei,Ablajan, Keyume,et al. Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(12):1953-1958.
APA	Guo, Na.,Yang, Dawei.,Ablajan, Keyume.,Niu, Xiaohong.,Fan, Bin.,...&Liu, Baidong.(2013).Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,36(12),1953-1958.
MLA	Guo, Na,et al."Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 36.12(2013):1953-1958.