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Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography
Fa, Yun1; Yang, Haiyan1; Ji, Chengshuai2; Cui, He3; Zhu, Xinshu4; Du, Juan5; Gao, Jun1
2013-10-10
发表期刊ANALYTICA CHIMICA ACTA
卷号798页码:97-102
摘要An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5-4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum. (C) 2013 Elsevier B. V. All rights reserved.
文章类型Article
关键词Ion Chromatography Valve Switching Amino Acids Carbohydrates Electrochemical Detector
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.aca.2013.08.033
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000324891500011
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6038
专题公共实验室
作者单位1.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.China Univ Petr, Qingdao Econ & Technol Dev Zone, Qingdao 266580, Peoples R China
3.Shandong Entry Exit Inspect & Quarantine Bur, Ctr Tech, Qingdao 266002, Peoples R China
4.Chinese Acad Sci, Metabol Grp, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
5.Qingdao Univ Sci & Technol, Coll Mat Sci & Engn, Qingdao 266042, Peoples R China
推荐引用方式
GB/T 7714
Fa, Yun,Yang, Haiyan,Ji, Chengshuai,et al. Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography[J]. ANALYTICA CHIMICA ACTA,2013,798:97-102.
APA Fa, Yun.,Yang, Haiyan.,Ji, Chengshuai.,Cui, He.,Zhu, Xinshu.,...&Gao, Jun.(2013).Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography.ANALYTICA CHIMICA ACTA,798,97-102.
MLA Fa, Yun,et al."Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography".ANALYTICA CHIMICA ACTA 798(2013):97-102.
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