Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography | |
Fa, Yun1; Yang, Haiyan1; Ji, Chengshuai2; Cui, He3; Zhu, Xinshu4; Du, Juan5; Gao, Jun1 | |
2013-10-10 | |
发表期刊 | ANALYTICA CHIMICA ACTA |
卷号 | 798页码:97-102 |
摘要 | An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5-4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum. (C) 2013 Elsevier B. V. All rights reserved. |
文章类型 | Article |
关键词 | Ion Chromatography Valve Switching Amino Acids Carbohydrates Electrochemical Detector |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1016/j.aca.2013.08.033 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000324891500011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6038 |
专题 | 公共实验室 |
作者单位 | 1.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.China Univ Petr, Qingdao Econ & Technol Dev Zone, Qingdao 266580, Peoples R China 3.Shandong Entry Exit Inspect & Quarantine Bur, Ctr Tech, Qingdao 266002, Peoples R China 4.Chinese Acad Sci, Metabol Grp, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 5.Qingdao Univ Sci & Technol, Coll Mat Sci & Engn, Qingdao 266042, Peoples R China |
推荐引用方式 GB/T 7714 | Fa, Yun,Yang, Haiyan,Ji, Chengshuai,et al. Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography[J]. ANALYTICA CHIMICA ACTA,2013,798:97-102. |
APA | Fa, Yun.,Yang, Haiyan.,Ji, Chengshuai.,Cui, He.,Zhu, Xinshu.,...&Gao, Jun.(2013).Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography.ANALYTICA CHIMICA ACTA,798,97-102. |
MLA | Fa, Yun,et al."Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography".ANALYTICA CHIMICA ACTA 798(2013):97-102. |
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