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Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases
Liu, Aiqiu1,2,3; Tan, Xiaoming1; Yao, Lun1,3; Lu, Xuefeng1
2013-08-01
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号97期号:15页码:7061-7071
摘要Although successful production of fatty alcohols in metabolically engineered Escherichia coli with heterologous expression of fatty acyl-CoA reductase has been reported, low biosynthetic efficiency is still a hurdle to be overcome. In this study, we examined the characteristics of two fatty acyl-CoA reductases encoded by Maqu_2220 and Maqu_2507 genes from Marinobacter aquaeolei VT8 on fatty alcohol production in E. coli. Fatty alcohols with diversified carbon chain length were obtained by co-expressing Maqu_2220 with different carbon chain length-specific acyl-ACP thioesterases. Both fatty acyl-CoA reductases displayed broad substrate specificities for C12-C18 fatty acyl chains in vivo. The optimized mutant strain of E. coli carrying the modified tesA gene and fadD gene from E. coli and Maqu_2220 gene from Marinobacter aquaeolei VT8 produced fatty alcohols at a remarkable level of 1.725 g/L under the fermentation condition.
文章类型Article
关键词Escherichia Coli Fatty Alcohol Fatty Acyl-coa Reductase Marinobacter Aquaeolei Vt8
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-013-5027-2
关键词[WOS]CARRIER PROTEIN THIOESTERASE ; COENZYME-A SYNTHETASE ; ACID PRODUCTION ; WAX PRODUCTION ; AQUAEOLEI VT8 ; BIOSYNTHESIS ; PURIFICATION ; OVERPRODUCTION ; CYANOBACTERIA ; ARABIDOPSIS
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000321632700040
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6031
专题微生物代谢工程研究组
作者单位1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Shandong Univ Weihai, Ocean Coll, Weihai 264209, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Liu, Aiqiu,Tan, Xiaoming,Yao, Lun,et al. Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(15):7061-7071.
APA Liu, Aiqiu,Tan, Xiaoming,Yao, Lun,&Lu, Xuefeng.(2013).Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(15),7061-7071.
MLA Liu, Aiqiu,et al."Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.15(2013):7061-7071.
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