| Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases | |
| Liu, Aiqiu1,2,3; Tan, Xiaoming1; Yao, Lun1,3; Lu, Xuefeng1 | |
| 2013-08-01 | |
| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 卷号 | 97期号:15页码:7061-7071 |
| 摘要 | Although successful production of fatty alcohols in metabolically engineered Escherichia coli with heterologous expression of fatty acyl-CoA reductase has been reported, low biosynthetic efficiency is still a hurdle to be overcome. In this study, we examined the characteristics of two fatty acyl-CoA reductases encoded by Maqu_2220 and Maqu_2507 genes from Marinobacter aquaeolei VT8 on fatty alcohol production in E. coli. Fatty alcohols with diversified carbon chain length were obtained by co-expressing Maqu_2220 with different carbon chain length-specific acyl-ACP thioesterases. Both fatty acyl-CoA reductases displayed broad substrate specificities for C12-C18 fatty acyl chains in vivo. The optimized mutant strain of E. coli carrying the modified tesA gene and fadD gene from E. coli and Maqu_2220 gene from Marinobacter aquaeolei VT8 produced fatty alcohols at a remarkable level of 1.725 g/L under the fermentation condition. |
| 文章类型 | Article |
| 关键词 | Escherichia Coli Fatty Alcohol Fatty Acyl-coa Reductase Marinobacter Aquaeolei Vt8 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00253-013-5027-2 |
| 关键词[WOS] | CARRIER PROTEIN THIOESTERASE ; COENZYME-A SYNTHETASE ; ACID PRODUCTION ; WAX PRODUCTION ; AQUAEOLEI VT8 ; BIOSYNTHESIS ; PURIFICATION ; OVERPRODUCTION ; CYANOBACTERIA ; ARABIDOPSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000321632700040 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6031 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Shandong Univ Weihai, Ocean Coll, Weihai 264209, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Aiqiu,Tan, Xiaoming,Yao, Lun,et al. Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(15):7061-7071. |
| APA | Liu, Aiqiu,Tan, Xiaoming,Yao, Lun,&Lu, Xuefeng.(2013).Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(15),7061-7071. |
| MLA | Liu, Aiqiu,et al."Fatty alcohol production in engineered E-coli expressing Marinobacter fatty acyl-CoA reductases".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.15(2013):7061-7071. |
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