| SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY | |
| Cui, Jiatao1,3; Zhang, Jingtao1; Zhu, Xinshu1,3; Bai, Fali2; Feng, Yingang1; Guan, Wenna2; Cui, Qiu1 | |
| 2013-11-22 | |
| 发表期刊 | ANALYTICAL LETTERS
 |
| 卷号 | 46期号:17页码:2767-2786 |
| 摘要 | A new protocol for metabolomic studies was developed by combining liquid chromatography-tandem mass spectrometry and isotope dilution mass spectrometry with universal C-13 labeled internal standards from Escherichia Coli. The multiple reaction monitoring mode of mass spectrometry was used for quantification. Forty-five water-soluble intracellular metabolites, including 20 amino acids, 16 organic acids (primarily from the tricarboxylic acid cycle), and 9 cofactors, were measured and 34 of them were successfully quantified using the C-13-labeled internal standards. The limit of detection, limit of quantification, precision, and linearity of the methods were evaluated. The methods were applied to the quantitative analysis of intracellular metabolites extracted from wild-type and ethanol-adapted strains of Clostridium thermocellum cultivated with and without ethanol stress, and all 34 metabolites including all 9 cofactors were successfully quantified. Further multivariate data analyses of the metabolic differences between wild-type and ethanol-adapted strains were performed on the quantitative data, which can help elucidate the metabolic mechanism behind ethanol adaptation in C. thermocellum. Supplemental materials are available for this article. Go to the publisher's online edition of Analytical Letters to view the supplemental file. |
| 文章类型 | Article |
| 关键词 | Clostridium Thermocellum Ethanol Tolerance Isotope Dilution Mass Spectrometry Liquid Chromatography-mass Spectrometry Water-soluble Metabolites |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1080/00032719.2013.811680 |
| 关键词[WOS] | TRICARBOXYLIC-ACID CYCLE ; BIOLOGICAL SAMPLES ; HUMAN PLASMA ; QUANTITATIVE-DETERMINATION ; INTRACELLULAR METABOLITES ; ELECTROSPRAY-IONIZATION ; HILIC-MS/MS ; PHASE ; EXTRACTION ; METABOLOMICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000325960300009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/5996 |
| 专题 | 代谢物组学研究组 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Jiatao,Zhang, Jingtao,Zhu, Xinshu,et al. SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY[J]. ANALYTICAL LETTERS,2013,46(17):2767-2786. |
| APA | Cui, Jiatao.,Zhang, Jingtao.,Zhu, Xinshu.,Bai, Fali.,Feng, Yingang.,...&Cui, Qiu.(2013).SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY.ANALYTICAL LETTERS,46(17),2767-2786. |
| MLA | Cui, Jiatao,et al."SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY".ANALYTICAL LETTERS 46.17(2013):2767-2786. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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