Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803

doi:10.1002/jobm.201200228

QIBEBT-IR > 微生物代谢工程研究组

	Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803
	Gao, Qianqian 1,2; Tan, Xiaoming 1; Lu, Xuefeng 1
	2013-10-01
发表期刊	JOURNAL OF BASIC MICROBIOLOGY
卷号	53 期号:10 页码:848-855
摘要	Free fatty acids are typically activated by thioesterification processes and catalyzed by the fatty acyl-CoA synthetase or fatty acyl-ACP synthetase. However, the routes for fatty acid activation in cyanobacteria are not well understood. In this investigation, the slr1609 gene, which encodes the fatty acid activation enzyme, was cloned from Synechocystis sp. PCC6803. This gene was identified by heterologous expression and in vitro enzymatic activity analyses. Different from previous reports stating that free fatty acids are only activated through the fatty acyl-ACP synthetases encoded by these genes in cyanobacteria, this gene was also proven to possess a fatty acyl-CoA synthetase activity, by in vitro enzymatic activity analyses and in vivo complementation experiments. The protein Slr1609 is located in both the cell membrane and the cytosol of Synechocystis sp. PCC6803. The differences in the transcriptional profiles between the wild type and the slr1609 deletion mutant strain were evaluated using microarray analyses. These analyses indicated that 299 differentially expressed genes are involved in fatty acid metabolism, photosynthesis, carbon fixation, stress tolerance and other metabolic processes. Our experiments demonstrate the observed compositional changes in the unsaturated fatty acids from the membrane lipids of the slr1609 deletion mutant when shifted from 30 to 24 degrees C.
文章类型	Article
关键词	Fatty Acid Activation Slr1609 Synechocystis Sp Pcc6803
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/jobm.201200228
关键词[WOS]	ACYL CARRIER PROTEIN ; SYNTHETASE ; CYANOBACTERIA ; EXPRESSION ; GENES ; PURIFICATION ; ARABIDOPSIS ; PCC-6803 ; MUTANTS ; COLI
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000325544500008
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/5993
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, Qianqian,Tan, Xiaoming,Lu, Xuefeng. Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803[J]. JOURNAL OF BASIC MICROBIOLOGY,2013,53(10):848-855.
APA	Gao, Qianqian,Tan, Xiaoming,&Lu, Xuefeng.(2013).Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803.JOURNAL OF BASIC MICROBIOLOGY,53(10),848-855.
MLA	Gao, Qianqian,et al."Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp PCC6803".JOURNAL OF BASIC MICROBIOLOGY 53.10(2013):848-855.