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Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production
Lin, Lu; Xu, Jian
2013-11-01
Source PublicationBIOTECHNOLOGY ADVANCES
Volume31Issue:6Pages:827-837
Abstract

Interest in thermophilic bacteria as live-cell catalysts in biofuel and biochemical industry has surged in recent years, due to their tolerance of high temperature and wide spectrum of carbon-sources that include cellulose. However their direct employment as microbial cellular factories in the highly demanding industrial conditions has been hindered by uncompetitive biofuel productivity, relatively low tolerance to solvent and osmic stresses, and limitation in genome engineering tools. In this work we review recent advances in dissecting and engineering the metabolic and regulatory networks of thermophilic bacteria for improving the traits of key interest in biofuel industry: cellulose degradation, pentose-hexose co-utilization, and tolerance of thermal, osmotic, and solvent stresses. Moreover, new technologies enabling more efficient genetic engineering of thermophiles were discussed, such as improved electroporation, ultrasound-mediated DNA delivery, as well as thermo-stable plasmids and functional selection systems. Expanded applications of such technological advancements in thermophilic microbes promise to substantiate a synthetic biology perspective, where functional parts, module, chassis, cells and consortia were modularly designed and rationally assembled for the many missions at industry and nature that demand the extraordinary talents of these extremophiles. (C) 2013 Elsevier Inc. All rights reserved.

SubtypeReview
KeywordThermophilic Bacteria Carbon Utilization Stress Response Microevolution Genetic Engineering Biofuels
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.biotechadv.2013.03.003
WOS KeywordGREEN FLUORESCENT PROTEIN ; HEAT-SHOCK RESPONSE ; IMPROVED ETHANOL TOLERANCE ; COMPLETE GENOME SEQUENCE ; CLOSTRIDIUM-THERMOCELLUM ; THERMUS-THERMOPHILUS ; ESCHERICHIA-COLI ; TRANSCRIPTIONAL ANALYSIS ; COMPATIBLE SOLUTES ; THERMOANAEROBACTER-ETHANOLICUS
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000324352700009
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/5984
Collection单细胞中心
AffiliationChinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, BioEnergy Genome Ctr,CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Lin, Lu,Xu, Jian. Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production[J]. BIOTECHNOLOGY ADVANCES,2013,31(6):827-837.
APA Lin, Lu,&Xu, Jian.(2013).Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production.BIOTECHNOLOGY ADVANCES,31(6),827-837.
MLA Lin, Lu,et al."Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production".BIOTECHNOLOGY ADVANCES 31.6(2013):827-837.
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