An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme | |
Lin, Jinzhong1; Lu, Jing1,2,3; Feng, Yingang4; Sun, Mengyi1; Ye, Keqiong1,2,3 | |
2013-10-01 | |
发表期刊 | PLOS BIOLOGY |
卷号 | 11期号:10页码:1 |
摘要 | A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain, are components of the 90S preribosome or the small subunit processome that conducts early processing of 18S rRNA. Here, we determine the cocrystal structure of Utp22 and Rrp7 complex at 1.97 angstrom resolution and the NMR structure of a C-terminal fragment of Rrp7, which is not visible in the crystal structure. The structure reveals that Utp22 surprisingly resembles a dimeric class I tRNA CCA-adding enzyme yet with degenerate active sites, raising an interesting evolutionary connection between tRNA and rRNA processing machineries. Rrp7 binds extensively to Utp22 using a deviant RNA recognition motif and an extended linker. Functional sites on the two proteins were identified by structure-based mutagenesis in yeast. We show that Rrp7 contains a flexible RNA-binding C-terminal tail that is essential for association with preribosomes. RNA-protein crosslinking shows that Rrp7 binds at the central domain of 18S rRNA and shares a neighborhood with two processing H/ACA snoRNAs snR30 and snR10. Depletion of snR30 prevents the stable assembly of Rrp7 into preribosomes. Our results provide insight into the evolutionary origin and functional context of Utp22 and Rrp7. |
文章类型 | Article |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1371/journal.pbio.1001669 |
关键词[WOS] | SMALL NUCLEOLAR RNA ; SMALL-SUBUNIT PROCESSOME ; SACCHAROMYCES-CEREVISIAE ; PRE-RIBOSOMES ; TRANSLATION INITIATION ; EUKARYOTIC RIBOSOME ; NUCLEAR EXPORT ; CROSS-LINKING ; YEAST SNR30 ; U3 SNORNA |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biochemistry & Molecular Biology ; Biology |
WOS记录号 | WOS:000330350700001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/5981 |
专题 | 代谢物组学研究组 |
作者单位 | 1.Natl Inst Biol Sci, Beijing, Peoples R China 2.Peking Union Med Coll, Grad Sch, Beijing 100021, Peoples R China 3.Chinese Acad Med Sci, Beijing 100730, Peoples R China 4.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Shangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Lin, Jinzhong,Lu, Jing,Feng, Yingang,et al. An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme[J]. PLOS BIOLOGY,2013,11(10):1. |
APA | Lin, Jinzhong,Lu, Jing,Feng, Yingang,Sun, Mengyi,&Ye, Keqiong.(2013).An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme.PLOS BIOLOGY,11(10),1. |
MLA | Lin, Jinzhong,et al."An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme".PLOS BIOLOGY 11.10(2013):1. |
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