Oligosphaera ethanolica gen. nov., sp nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov in the phylum Lentisphaerae

doi:10.1099/ijs.0.039545-0

QIBEBT-IR > 工业生物燃气研究组

	Oligosphaera ethanolica gen. nov., sp nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov in the phylum Lentisphaerae
	Qiu, Yan-Ling1,2 ; Muramatsu, Mizuho 3; Hanada, Satoshi 4; Kamagata, Yoichi 3; Guo, Rong-Bo1 ; Sekiguchi, Yuji 2
	2013-02-01
发表期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷号	63 页码:533-539
摘要	A mesophilic, obligately anaerobic, carbohydrate-fermenting bacterium, designated 8KG-4(T), was isolated from an upflow anaerobic sludge blanket reactor treating high-strength organic wastewater from salted vegetable production processes. Cells of strain 8KG-4(T) were non-motile, spherical and 0.7-1.5 mu m in diameter (mean, 1.0 mu m). Spore formation was not observed under any culture conditions tested. The strain grew optimally at 37 degrees C (range for growth 25-40 degrees C) and pH 7.0 (range, pH 6.5-7.5), and could grow fermentatively on glucose, ribose, xylose, galactose and sucrose. The main end products of glucose fermentation were acetate, ethanol and hydrogen. Organic acids, alcohols and amino acids were not utilized for growth. Yeast extract was not required for growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite and Fe(III) nitrilotriacetate were not used as terminal electron acceptors. The G+C content of the genomic DNA was 61.1 mol%. 16S rRNA gene sequence analysis revealed that the isolate represented a previously uncultured lineage at the subphylum level within the phylum Lentisphaerae known as 'WWE2 subgroup I'. The major cellular fatty acids were anteiso-C-15:0, iso-C-16:0, C-16:0 and anteiso-C-17:0. Respiratory quinones were not detected. The most abundant polar lipid of strain 8KG-4(T) was phosphatidylethanolamine. A novel genus and species, Oligosphaera ethanolica gen. nov., sp. nov., is proposed to accommodate strain 8KG-4(T) (=JCM 17152(T)=DSM 24202(T) =CGMCC 1.5160(T)). In addition, we formally propose Oligosphaeria classis nov. and the subordinate taxa Oligosphaerales order nov. and Oligosphaeraceae fam. nov.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1099/ijs.0.039545-0
关键词[WOS]	ENVIRONMENT ; DIVERSITY
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000317170400022
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/5980
专题	工业生物燃气研究组
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 2.Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan 3.Natl Inst Adv Ind Sci & Technol, Bioproc Res Inst, Sapporo, Hokkaido 0628517, Japan 4.Natl Inst Adv Ind Sci & Technol, Bioproc Res Inst, Tsukuba, Ibaraki 3058566, Japan
推荐引用方式 GB/T 7714	Qiu, Yan-Ling,Muramatsu, Mizuho,Hanada, Satoshi,et al. Oligosphaera ethanolica gen. nov., sp nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov in the phylum Lentisphaerae[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2013,63:533-539.
APA	Qiu, Yan-Ling,Muramatsu, Mizuho,Hanada, Satoshi,Kamagata, Yoichi,Guo, Rong-Bo,&Sekiguchi, Yuji.(2013).Oligosphaera ethanolica gen. nov., sp nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov in the phylum Lentisphaerae.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,63,533-539.
MLA	Qiu, Yan-Ling,et al."Oligosphaera ethanolica gen. nov., sp nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov in the phylum Lentisphaerae".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63(2013):533-539.