Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116 | |
Gao, Mang1; Song, Xiaojin1; Feng, Yingang1; Li, Wenli3; Cui, Qiu1,2 | |
2013-03-01 | |
发表期刊 | JOURNAL OF OLEO SCIENCE |
卷号 | 62期号:3页码:143-151 |
摘要 | A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to Aurantiochytrium species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28 degrees C. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial fermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L-1, 71.09% of the DCW, and 17.42 g L-1 (34.79% of the TFA), respectively. The results show that Aurantiochytrium sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products. |
文章类型 | Article |
关键词 | Aurantiochytrium Sp. Biomass Docosahexaenoic Acid Heterotrophic Marine Algae Lipid |
WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
DOI | 10.5650/jos.62.143 |
关键词[WOS] | CARDIAC SUDDEN-DEATH ; SCHIZOCHYTRIUM-LIMACINUM ; CULTURE-CONDITIONS ; FATTY-ACIDS ; OPTIMIZATION ; GROWTH ; BIOSYNTHESIS ; PREVENTION ; MANGROVEI |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry ; Food Science & Technology |
项目资助者 | Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-J-10 ; KGCX2-YW-374-12) |
WOS类目 | Chemistry, Applied ; Food Science & Technology |
WOS记录号 | WOS:000315362000004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/5949 |
专题 | 代谢物组学研究组 |
作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 3.Ocean Univ China, Key Lab Marine Drugs, Minist Educ, Sch Med & Pharm, Qingdao, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Mang,Song, Xiaojin,Feng, Yingang,et al. Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116[J]. JOURNAL OF OLEO SCIENCE,2013,62(3):143-151. |
APA | Gao, Mang,Song, Xiaojin,Feng, Yingang,Li, Wenli,&Cui, Qiu.(2013).Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116.JOURNAL OF OLEO SCIENCE,62(3),143-151. |
MLA | Gao, Mang,et al."Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp SD116".JOURNAL OF OLEO SCIENCE 62.3(2013):143-151. |
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