Raman Activated Cell Ejection for Isolation of Single Cells

doi:10.1021/ac403107p

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	Raman Activated Cell Ejection for Isolation of Single Cells
	Wang, Yun1,2 ; Ji, Yuetong1,2 ; Wharfe, Emma S.3; Meadows, Roger S.4; March, Peter 4; Goodacre, Royston 5; Xu, Jian1,2 ; Huang, Wei E.3
	2013-11-19
发表期刊	ANALYTICAL CHEMISTRY
卷号	85 期号:22 页码:10697-10701
摘要	We have optimized a Raman microscope to obtain a single cell Raman spectrum (SCRS) with 0.1 s acquisition time. SCRS with such short acquisition time has sufficient discriminatory ability and spectral reproducibility to differentiate cells incorporated with C-13 and N-15 and to classify five different types of bacteria isolated from the oral cavity. We also developed Raman activated cell ejection (RACE) that is assisted by laser induced forward transfer (LIFT). We have shown, for the first time, that the single cells of interest can be identified and then accurately isolated from complex microbial communities based on their SCRS. This approach can be used to sort single cells of target traits from complex samples (e.g., biofilms, soils, sludge, tissues).
文章类型	Article
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1021/ac403107p
关键词[WOS]	IN-SITU HYBRIDIZATION ; SPECTROSCOPY ; MICROSPECTROSCOPY ; BACTERIA
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000327360900013
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/5948
专题	单细胞中心组群
作者单位	1.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England 4.Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England 5.Univ Manchester, Manchester Inst Biotechnol, Sch Chem, Manchester M1 7DN, Lancs, England
推荐引用方式 GB/T 7714	Wang, Yun,Ji, Yuetong,Wharfe, Emma S.,et al. Raman Activated Cell Ejection for Isolation of Single Cells[J]. ANALYTICAL CHEMISTRY,2013,85(22):10697-10701.
APA	Wang, Yun.,Ji, Yuetong.,Wharfe, Emma S..,Meadows, Roger S..,March, Peter.,...&Huang, Wei E..(2013).Raman Activated Cell Ejection for Isolation of Single Cells.ANALYTICAL CHEMISTRY,85(22),10697-10701.
MLA	Wang, Yun,et al."Raman Activated Cell Ejection for Isolation of Single Cells".ANALYTICAL CHEMISTRY 85.22(2013):10697-10701.