Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical

	Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical
	Xiangzhao Mao; Xiaotong Liang; Shu Wang; Wei Dong; Yanlong Xing; Hualei Wang; Lizhong Guo1; Dongzhi Wei
	2010
发表期刊	African Journal of Biotechnology
ISSN	1684–5315
卷号	9 期号:8 页码:1180-1189
摘要	Optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5 was carried out in the shake flasks using two kinds of statistical methods. Four variables, namely glucose, tryptone, yeast extract and sodium chloride, were found to influence DDase production significantly by the Plackett-Burman screening. A four-factor five-level central composite design (CCD) was chosen to explain the combined effects of the four medium constituents. The optimum medium consisted of glucose (17.670 g/L), maltobiose (30 g/L), tryptone (12.198 g/L), yeast extract (13.528 g/L), ammonium nitrate (15 g/L), copper sulfate (0.01 g/L), zinc sulfate (0.01 g/L), and sodium chloride (0.009 g/L); the initial pH 6.0 was set prior to sterilization. The DDase yield obtained from optimized medium increased by 17-fold (0.238 U/mL) or so. Under these optimal conditions, the experimental values agreed with the predicted values, indicating that the chosen method of optimization of medium composition was efficient, relatively simple, time reducing and material saving.
关键词	Dextran Dextrinase Gluconobacter Oxydans Medium Optimization Plackett-burmandesign Central Composite Design
学科领域	储氢及新型纳孔材料
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/548
专题	储氢及新型纳孔材料团队（过去）
推荐引用方式 GB/T 7714	Xiangzhao Mao,Xiaotong Liang,Shu Wang,et al. Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical[J]. African Journal of Biotechnology,2010,9(8):1180-1189.
APA	Xiangzhao Mao.,Xiaotong Liang.,Shu Wang.,Wei Dong.,Yanlong Xing.,...&Dongzhi Wei.(2010).Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical.African Journal of Biotechnology,9(8),1180-1189.
MLA	Xiangzhao Mao,et al."Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical".African Journal of Biotechnology 9.8(2010):1180-1189.

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