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Problems with the microbial production of butanol
Zheng, Yan-Ning1; Li, Liang-Zhi1; Xian, Mo1; Ma, Yu-Jiu1; Yang, Jian-Ming1; Xu, Xin1; He, Dong-Zhi2
2009-09-01
Source PublicationJOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSNISSN : 1476-5535
Volume36Issue:9Pages:1127-1138
AbstractWith the incessant fluctuations in oil prices and increasing stress from environmental pollution, renewed attention is being paid to the microbial production of biofuels from renewable sources. As a gasoline substitute, butanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. A variety of cheap substrates have been successfully applied in the production of biobutanol, highlighting the commercial potential of biobutanol development. In this review, in order to better understand the process of acetone-butanol-ethanol production, traditional clostridia fermentation is discussed. Sporulation is probably induced by solvent formation, and the molecular mechanism leading to the initiation of sporulation and solventogenesis is also investigated. Different strategies are employed in the metabolic engineering of clostridia that aim to enhancing solvent production, improve selectivity for butanol production, and increase the tolerance of clostridia to solvents. However, it will be hard to make breakthroughs in the metabolic engineering of clostridia for butanol production without gaining a deeper understanding of the genetic background of clostridia and developing more efficient genetic tools for clostridia. Therefore, increasing attention has been paid to the metabolic engineering of E. coli for butanol production. The importation and expression of a non-clostridial butanol-producing pathway in E. coli is probably the most promising strategy for butanol biosynthesis. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to reduce the cost of butanol recovery. Gas stripping is the best technique for butanol recovery found so far.; With the incessant fluctuations in oil prices and increasing stress from environmental pollution, renewed attention is being paid to the microbial production of biofuels from renewable sources. As a gasoline substitute, butanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. A variety of cheap substrates have been successfully applied in the production of biobutanol, highlighting the commercial potential of biobutanol development. In this review, in order to better understand the process of acetone-butanol-ethanol production, traditional clostridia fermentation is discussed. Sporulation is probably induced by solvent formation, and the molecular mechanism leading to the initiation of sporulation and solventogenesis is also investigated. Different strategies are employed in the metabolic engineering of clostridia that aim to enhancing solvent production, improve selectivity for butanol production, and increase the tolerance of clostridia to solvents. However, it will be hard to make breakthroughs in the metabolic engineering of clostridia for butanol production without gaining a deeper understanding of the genetic background of clostridia and developing more efficient genetic tools for clostridia. Therefore, increasing attention has been paid to the metabolic engineering of E. coli for butanol production. The importation and expression of a non-clostridial butanol-producing pathway in E. coli is probably the most promising strategy for butanol biosynthesis. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to reduce the cost of butanol recovery. Gas stripping is the best technique for butanol recovery found so far.
SubtypeReview
KeywordButanol Fermentation Metabolic Engineering Clostridia E. Coli Recovery Techniques
Subject Area生物基化学品
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-009-0609-9
WOS KeywordCLOSTRIDIUM-BEIJERINCKII BA101 ; ACETOBUTYLICUM ATCC 824 ; INCREASED SOLVENT PRODUCTION ; FACTOR HOMOLOGOUS GENES ; FERMENTATION PROCESS ; ACETONE FORMATION ; DOWN-REGULATION ; TRANSCRIPTIONAL REPRESSOR ; SYNTHETIC GENES ; REPORTER GENE
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000269193600001
Citation statistics
Cited Times:169[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/460
Collection材料生物技术研究中心
Affiliation1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China
2.Dalian Polytech Univ, Coll Profess Technol, Dalian 116221, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Yan-Ning,Li, Liang-Zhi,Xian, Mo,et al. Problems with the microbial production of butanol[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2009,36(9):1127-1138.
APA Zheng, Yan-Ning.,Li, Liang-Zhi.,Xian, Mo.,Ma, Yu-Jiu.,Yang, Jian-Ming.,...&He, Dong-Zhi.(2009).Problems with the microbial production of butanol.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,36(9),1127-1138.
MLA Zheng, Yan-Ning,et al."Problems with the microbial production of butanol".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 36.9(2009):1127-1138.
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