| Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology | |
Li, Liang-zhi1,2; Zheng, Hui1; Xian, Mo2
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| 2010-05-01 | |
| 发表期刊 | JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
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| ISSN | ISSN: 1876-1070 |
| 卷号 | 41期号:3页码:252-258 |
| 摘要 | Plackett-Burman multifactorial design and the consequent response surface methodology (RSM) were used to improve the streptolydigin yield in a newly isolated strain Streptomyces lydicus AS 4.2501 with a dramatic streptolydigin increase of 18.6%. Also propionate's role as a precursor for the streptolydigin biosynthesis was for the first time practically validated. The results from the Plackett-Burman design showed that starch, peptone and K(2)HPO(4) were influential factors to the biosynthesis of streptolydigin. Consequent analysis with RSM not only detected the interactions among these important factors, but also established two models referring to the streptolydigin titer and biomass, respectively. Additionally, the relationship between the production of streptolydigin and the growth of mycelia was first discussed using statistical methods, which is of great help to the overproduction of the related secondary metabolites.; Plackett-Burman multifactorial design and the consequent response surface methodology (RSM) were used to improve the streptolydigin yield in a newly isolated strain Streptomyces lydicus AS 4.2501 with a dramatic streptolydigin increase of 18.6%. Also propionate's role as a precursor for the streptolydigin biosynthesis was for the first time practically validated. The results from the Plackett-Burman design showed that starch, peptone and K(2)HPO(4) were influential factors to the biosynthesis of streptolydigin. Consequent analysis with RSM not only detected the interactions among these important factors, but also established two models referring to the streptolydigin titer and biomass, respectively. Additionally, the relationship between the production of streptolydigin and the growth of mycelia was first discussed using statistical methods, which is of great help to the overproduction of the related secondary metabolites. (C) 2010 Published by Elsevier B.V. on behalf of Taiwan Institute of Chemical Engineers. |
| 文章类型 | Article |
| 关键词 | Response Surface Methodology Fermentation Streptolydigin Optimization Streptomyces Lydicus |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Technology |
| DOI | 10.1016/j.jtice.2009.11.001 |
| 关键词[WOS] | RNA-POLYMERASE ; LACTIC-ACID ; BIOSYNTHESIS ; FERMENTATION ; DESIGN ; INHIBITION ; PROPIONATE ; LIPASE ; CARBON ; PH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Engineering |
| WOS类目 | Engineering, Chemical |
| WOS记录号 | WOS:000278239900002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/458 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Dept Pharmaceut Engn, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Liang-zhi,Zheng, Hui,Xian, Mo. Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology[J]. JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS,2010,41(3):252-258. |
| APA | Li, Liang-zhi,Zheng, Hui,&Xian, Mo.(2010).Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology.JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS,41(3),252-258. |
| MLA | Li, Liang-zhi,et al."Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology".JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS 41.3(2010):252-258. |
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