| Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401 | |
| Yu-Cai He; Jian-He Xu; Jin-Huan Su; Li Zhou | |
| 2009 | |
| 发表期刊 | Applied Biochemistry and Biotechnology
 |
| 卷号 | 160期号:5页码:1428-1440 |
| 摘要 | Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and Cu(2+), respectively. Glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mM glycolonitrile in the mode of sequential addition during the cultivation of Alcaligenes sp. ECU0401 in a 5-L jar fermenter. Fifty micromolars of glycolonitrile could be hydrolyzed in a yield of 94.1% by resting cells after 36 h. The microbial nitrilase system could hydrolyze various nitriles with high activity, and no amidase activity and glycolic acid were observed in hydrolyzing glycolamide. It significantly exhibited high enantioselectivity in the hydrolysis of mandelonitrile and 2-chloromandelonitrile (> 99.9% e.e. (p) ). Efficient biocatalyst recycling was achieved as a result of immobilization in glutaraldehyde/polyethylenimine cross-linked carrageenan with immobilized cells exhibiting a biocatalyst productivity of 1,042.2 g glycolic acid per gram dry cell weight after 29 batch recycles. |
| 关键词 | Nitrilase Alcaligenes Sp. Ecu0401 Glycolic Acid Glycolonitrile Immobilization |
| 学科领域 | 生物基化学品 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/444 |
| 专题 | 生物基材料组群 |
| 推荐引用方式 GB/T 7714 | Yu-Cai He,Jian-He Xu,Jin-Huan Su,et al. Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401[J]. Applied Biochemistry and Biotechnology,2009,160(5):1428-1440. |
| APA | Yu-Cai He,Jian-He Xu,Jin-Huan Su,&Li Zhou.(2009).Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401.Applied Biochemistry and Biotechnology,160(5),1428-1440. |
| MLA | Yu-Cai He,et al."Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401".Applied Biochemistry and Biotechnology 160.5(2009):1428-1440. |
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