| Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein | |
Su, Yu-Jie1,2 ; Lin, Jian-Qun1; Lin, Jian-Qiang1; Hao, Dong-Hui1
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| 2009-09-01 | |
| 发表期刊 | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
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| ISSN | 1226-8372 |
| 卷号 | 14期号:5页码:565-570 |
| 摘要 | The recombinant Escherichia coli (E. coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E coli and synthesized using chemical method. The vector of pGEX_4T_1 was used and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E. coli increased more than 3-fold from 76.3 to 319.6 mu g/g dry cells compared with the control. The conditions of 50 mu M of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E. coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water.; The recombinant Escherichia coli (E. coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E coli and synthesized using chemical method. The vector of pGEX_4T_1 was used and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E. coli increased more than 3-fold from 76.3 to 319.6 mu g/g dry cells compared with the control. The conditions of 50 mu M of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E. coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water. (C) KSBB |
| 文章类型 | Article |
| 关键词 | Metallothionein Arsenic Bioaccumulation Bioremediation Heavy Metal |
| 学科领域 | 文献情报 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12257-008-0197-y |
| 关键词[WOS] | BACTERIA ; BIOREMEDIATION ; CELLS ; ACCUMULATION ; BIOSORPTION ; REMOVAL ; BINDING ; PROTEIN ; MARINE ; METALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000271405300003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/378 |
| 专题 | 规划战略与信息中心 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Yu-Jie,Lin, Jian-Qun,Lin, Jian-Qiang,et al. Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2009,14(5):565-570. |
| APA | Su, Yu-Jie,Lin, Jian-Qun,Lin, Jian-Qiang,&Hao, Dong-Hui.(2009).Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,14(5),565-570. |
| MLA | Su, Yu-Jie,et al."Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 14.5(2009):565-570. |
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