| Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli | |
Yang, Jianming1; Zhao, Guang1; Sun, Yuanzhang2; Zheng, Yanning1; Jiang, Xinglin1; Liu, Wei1; Xian, Mo1
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| 2012 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| 卷号 | 104期号:104页码:642-647 |
| 摘要 | In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in E. coli has been provided. (C) 2011 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Isoprene Mva Pathway Isoprene Synthase Escherichia Coli |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2011.10.042 |
| 关键词[WOS] | LYCOPENE PRODUCTION ; MEVALONATE PATHWAY ; BIOSYNTHESIS ; EXPRESSION ; EMISSION ; RUBBER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000301155800086 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1752 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Shandong Polytech Univ, Sch Food & Bioengn, Jinan 250353, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jianming,Zhao, Guang,Sun, Yuanzhang,et al. Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2012,104(104):642-647. |
| APA | Yang, Jianming.,Zhao, Guang.,Sun, Yuanzhang.,Zheng, Yanning.,Jiang, Xinglin.,...&Xian, Mo.(2012).Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli.BIORESOURCE TECHNOLOGY,104(104),642-647. |
| MLA | Yang, Jianming,et al."Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli".BIORESOURCE TECHNOLOGY 104.104(2012):642-647. |
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