| Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli | |
Wang, Qi1,2; Liu, Changshui1,2; Xian, Mo1 ; Zhang, Yongguang1; Zhao, Guang1
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| 2012-08-01 | |
| 发表期刊 | JOURNAL OF MICROBIOLOGY
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| 卷号 | 50期号:4页码:693-697 |
| 摘要 | Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria. |
| 文章类型 | Article |
| 关键词 | Poly(3-hydroxypropionate) Malonyl-coa Reductase Propionyl-coenzyme a Synthetase Polyhydroxyalkanoate Synthase RE.ombinant E. Coli |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12275-012-2234-y |
| 关键词[WOS] | PSEUDOMONAS-OLEOVORANS ; RALSTONIA-EUTROPHA ; POLYESTERS ; COENZYME ; POLYHYDROXYALKANOATES ; 3-HYDROXYPROPIONATE ; ACCUMULATION ; SYNTHETASE ; PUTIDA ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000307996700022 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1749 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Liu, Changshui,Xian, Mo,et al. Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli[J]. JOURNAL OF MICROBIOLOGY,2012,50(4):693-697. |
| APA | Wang, Qi,Liu, Changshui,Xian, Mo,Zhang, Yongguang,&Zhao, Guang.(2012).Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli.JOURNAL OF MICROBIOLOGY,50(4),693-697. |
| MLA | Wang, Qi,et al."Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli".JOURNAL OF MICROBIOLOGY 50.4(2012):693-697. |
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| Biosynthetic Pathway（315KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
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