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Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria
Gao, Zhengxu1,2; Zhao, Hui1; Li, Zhimin1; Tan, Xiaoming1; Lu, Xuefeng1
2012-12-01
发表期刊ENERGY & ENVIRONMENTAL SCIENCE
卷号5期号:12页码:9857-9865
摘要The rapidly growing demand for energy and the environmental concerns about carbon dioxide emissions make the development of renewable biofuels more and more attractive. Tremendous academic and industrial efforts have been made to produce bioethanol, which is one major type of biofuel. The current production of bioethanol is limited for commercialization because of issues with food competition (from food-based biomass) or cost effectiveness (from lignocellulose-based biomass). In this report we applied a consolidated bioprocessing strategy to integrate photosynthetic biomass production and microbial conversion producing ethanol together into the photosynthetic bacterium, Synechocystis sp. PCC6803, which can directly convert carbon dioxide to ethanol in one single biological system. A Synechocystis sp. PCC6803 mutant strain with significantly higher ethanol-producing efficiency (5.50 g L-1, 212 mg L-1 day(-1)) compared to previous research was constructed by genetically introducing pyruvate decarboxylase from Zymomonas mobilis and overexpressing endogenous alcohol dehydrogenase through homologous recombination at two different sites of the chromosome, and disrupting the biosynthetic pathway of poly-beta-hydroxybutyrate. In total, nine alcohol dehydrogenases from different cyanobacterial strains were cloned and expressed in E. coli to test ethanol-producing efficiency. The effects of different culturing conditions including tap water, metal ions, and anoxic aeration on ethanol production were evaluated.
文章类型Article
学科领域生物代谢工程
WOS标题词Science & Technology ; Physical Sciences ; Technology ; Life Sciences & Biomedicine
DOI10.1039/c2ee22675h
关键词[WOS]SYNECHOCYSTIS SP PCC6803 ; BIOFUELS PRODUCTION ; ZYMOMONAS-MOBILIS ; DEHYDROGENASE ; PURIFICATION ; BIODIESEL ; BIOMASS ; ENHANCE ; FUTURE ; FUELS
收录类别SCI
语种英语
WOS研究方向Chemistry ; Energy & Fuels ; Engineering ; Environmental Sciences & Ecology
WOS类目Chemistry, Multidisciplinary ; Energy & Fuels ; Engineering, Chemical ; Environmental Sciences
WOS记录号WOS:000311970300021
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1737
专题微生物代谢工程团队
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Gao, Zhengxu,Zhao, Hui,Li, Zhimin,et al. Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria[J]. ENERGY & ENVIRONMENTAL SCIENCE,2012,5(12):9857-9865.
APA Gao, Zhengxu,Zhao, Hui,Li, Zhimin,Tan, Xiaoming,&Lu, Xuefeng.(2012).Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria.ENERGY & ENVIRONMENTAL SCIENCE,5(12),9857-9865.
MLA Gao, Zhengxu,et al."Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria".ENERGY & ENVIRONMENTAL SCIENCE 5.12(2012):9857-9865.
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