| Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli | |
| Bao, Xue1,2; Liu, Aiqiu1,2; Lu, Xuefeng1; Li, Jian-Jun1 | |
| 2012-08-01 | |
| 发表期刊 | BIOTECHNOLOGY LETTERS
 |
| 卷号 | 34期号:8页码:1537-1543 |
| 摘要 | The vpl2 gene, encoding versatile peroxidase (VP) from Pleurotus eryngii, was synthesized with codon optimization and cloned into vector-pET-32a(+) and over-expressed in Escherichia coli BL21(DE3). An active peroxidase fused to the thioredoxin-hexahistidine tag was directly obtained by IPTG induction in the presence of hemin. Most of over-expressed protein was in the soluble form, and was purified on a nickel column with > 85 % purity at a yield of 12.5 mg/l. The purified fusion protein, having an Rz value (A(407)/A(280), a measure of hemin content of the peroxidases) of 1.2, oxidized ABTS veratryl alcohol, Mn2+, and Reactive Black 5. Activity of the enzyme increased after removing the tag. It lost only 5 % of its activity in 6.4 mM H2O2. This is the first report on direct over-expression of active VP in E. coli. |
| 文章类型 | Article |
| 关键词 | Escherichia Coli Hydrogen Peroxide Stability Overexpression Pleurotus Eryngii Peroxidase |
| 学科领域 | 生物代谢工程 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10529-012-0940-5 |
| 关键词[WOS] | PHANEROCHAETE-CHRYSOSPORIUM ; MANGANESE PEROXIDASE ; RECOMBINANT ; INACTIVATION ; MUTAGENESIS ; ENZYME ; PH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000306789200019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1736 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Xue,Liu, Aiqiu,Lu, Xuefeng,et al. Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2012,34(8):1537-1543. |
| APA | Bao, Xue,Liu, Aiqiu,Lu, Xuefeng,&Li, Jian-Jun.(2012).Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli.BIOTECHNOLOGY LETTERS,34(8),1537-1543. |
| MLA | Bao, Xue,et al."Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli".BIOTECHNOLOGY LETTERS 34.8(2012):1537-1543. |
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