| Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application | |
Wang, Hongwei1,2,3; Lang, Qiaolin1,2 ; Li, Liang1,2; Liang, Bo1,2; Tang, Xiangjiang1,2; Kong, Lingrang3; Mascini, Marco4; Liu, Aihua1,2
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| 2013-06-18 | |
| 发表期刊 | ANALYTICAL CHEMISTRY
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| 卷号 | 85期号:12页码:6107-6112 |
| 摘要 | The display of glucose oxidase (GOx) on yeast cell surface using a-agglutinin as an anchor motif was successfully developed. Both the immunochemical analysis and enzymatic assay showed that active GOx was efficiently expressed and translocated on the cell surface. Compared with conventional GOx, the yeast cell surface that displayed GOx (GOx-yeast) demonstrated excellent enzyme properties, such as good stability within a wide pH range (pH 3.5-11.5), good therrnostability (retaining over 94.8% enzyme activity at 52 degrees C and 84.2% enzyme activity at 56 degrees C), and high D-glucose specificity. In addition, direct electrochemistry was achieved at a GOx-yeast/multiwalled-carbon-nanotube modified electrode, suggesting that the host cell of yeast did not have any adverse effect on the electrocatalytic property of the recombinant GOx. Thus, a novel electrochemical glucose biosensor based on this GOx-yeast was developed. The as-prepared biosensor was linear with the concentration of D-glucose within the range of 0.1-14 mM and a low detection limit of 0.05 mM (signal-to-noise ratio of S/N = 3). Moreover, the as-prepared biosensor is stable, specific, reproducible, simple, and cost-effective, which can be applicable for real sample detection. The proposed strategy to construct robust GOx-yeast may be applied to explore other oxidase-displaying-system-based whole-cell biocatalysts, which can find broad potential application in biosensors,, bioenergy, and industrial catalysis. |
| 文章类型 | Article |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1021/ac400979r |
| 关键词[WOS] | ASPERGILLUS-NIGER ; SACCHAROMYCES-CEREVISIAE ; ELECTRON-TRANSFER ; DEHYDROGENASE ; EXPRESSION ; XYLOSE ; BIOSENSORS ; BACTERIA ; GENE ; IMMOBILIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000320749200069 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1692 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 4.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy |
| 推荐引用方式 GB/T 7714 | Wang, Hongwei,Lang, Qiaolin,Li, Liang,et al. Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application[J]. ANALYTICAL CHEMISTRY,2013,85(12):6107-6112. |
| APA | Wang, Hongwei.,Lang, Qiaolin.,Li, Liang.,Liang, Bo.,Tang, Xiangjiang.,...&Liu, Aihua.(2013).Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application.ANALYTICAL CHEMISTRY,85(12),6107-6112. |
| MLA | Wang, Hongwei,et al."Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application".ANALYTICAL CHEMISTRY 85.12(2013):6107-6112. |
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