| Metabolic Adaption of Ethanol-Tolerant Clostridium thermocellum | |
| Zhu, Xinshu1,3; Cui, Jiatao1,3; Feng, Yingang1; Fa, Yun2; Zhang, Jingtao1; Cui, Qiu1 | |
| 2013-07-30 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 8期号:7 |
| 摘要 | Clostridium thermocellum is a major candidate for bioethanol production via consolidated bioprocessing. However, the low ethanol tolerance of the organism dramatically impedes its usage in industry. To explore the mechanism of ethanol tolerance in this microorganism, systematic metabolomics was adopted to analyse the metabolic phenotypes of a C. thermocellum wild-type (WT) strain and an ethanol-tolerant strain cultivated without (ET0) or with (ET3) 3% (v/v) exogenous ethanol. Metabolomics analysis elucidated that the levels of numerous metabolites in different pathways were changed for the metabolic adaption of ethanol-tolerant C. thermocellum. The most interesting phenomenon was that cellodextrin was significantly more accumulated in the ethanol-tolerant strain compared with the WT strain, although cellobiose was completely consumed in both the ethanol-tolerant and wild-type strains. These results suggest that the cellodextrin synthesis was active, which might be a potential mechanism for stress resistance. Moreover, the overflow of many intermediate metabolites, which indicates the metabolic imbalance, in the ET0 cultivation was more significant than in the WT and ET3 cultivations. This indicates that the metabolic balance of the ethanol-tolerant strain was adapted better to the condition of ethanol stress. This study provides additional insight into the mechanism of ethanol tolerance and is valuable for further metabolic engineering aimed at higher bioethanol production. ; Clostridium thermocellum is a major candidate for bioethanol production via consolidated bioprocessing. However, the low ethanol tolerance of the organism dramatically impedes its usage in industry. To explore the mechanism of ethanol tolerance in this microorganism, systematic metabolomics was adopted to analyse the metabolic phenotypes of a C. thermocellum wild-type (WT) strain and an ethanol-tolerant strain cultivated without (ET0) or with (ET3) 3% (v/v) exogenous ethanol. Metabolomics analysis elucidated that the levels of numerous metabolites in different pathways were changed for the metabolic adaption of ethanol-tolerant C. thermocellum. The most interesting phenomenon was that cellodextrin was significantly more accumulated in the ethanol-tolerant strain compared with the WT strain, although cellobiose was completely consumed in both the ethanol-tolerant and wild-type strains. These results suggest that the cellodextrin synthesis was active, which might be a potential mechanism for stress resistance. Moreover, the overflow of many intermediate metabolites, which indicates the metabolic imbalance, in the ET0 cultivation was more significant than in the WT and ET3 cultivations. This indicates that the metabolic balance of the ethanol-tolerant strain was adapted better to the condition of ethanol stress. This study provides additional insight into the mechanism of ethanol tolerance and is valuable for further metabolic engineering aimed at higher bioethanol production. |
| 文章类型 | Article |
| 学科领域 | 代谢物组学 |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0070631 |
| 关键词[WOS] | ATCC 27405 ; FERMENTATION ; NMR ; CELLOBIOSE ; CELLULOSE ; BACTERIA ; STRESS ; CELLODEXTRINS ; MECHANISMS ; MEMBRANES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000323114200096 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1691 |
| 专题 | 代谢物组学研究组 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xinshu,Cui, Jiatao,Feng, Yingang,et al. Metabolic Adaption of Ethanol-Tolerant Clostridium thermocellum[J]. PLOS ONE,2013,8(7). |
| APA | Zhu, Xinshu,Cui, Jiatao,Feng, Yingang,Fa, Yun,Zhang, Jingtao,&Cui, Qiu.(2013).Metabolic Adaption of Ethanol-Tolerant Clostridium thermocellum.PLOS ONE,8(7). |
| MLA | Zhu, Xinshu,et al."Metabolic Adaption of Ethanol-Tolerant Clostridium thermocellum".PLOS ONE 8.7(2013). |
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