| Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor | |
Liang, Bo1,2 ; Li, Liang1,2,3; Tang, Xiangliang1,2; Lang, Qiaolin1,2; Wang, Hongwei1,2; Li, Feng3; Shi, Jianguo4; Shen, Wei5; Palchetti, Ilaria6; Mascini, Marco6; Liu, Aihua1,2
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| 2013-07-15 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| 卷号 | 45期号:2013页码:19-24 |
| 摘要 | A genetically engineered Escherichia coil (E. coil) strain displaying glucose dehydrogenase (GDH) with ice-nucleation protein (INP) as the anchoring motif was first constructed. The surface localization and functionality of the fusion protein containing GDH were verified by SDS-PAGE, Western blotting and enzymatic activity assay. The fusion of INP had no effects on the functionality of GDH cofactor binding domain. The activity assay showed that 74.6% of the cell lysate GDH activity was detected in the outer membrane fractions. Compared with the crude enzyme solution from E. coli expressing intracellular GDH, the GDH-displaying bacteria (GDH-bacteria) was stable within pH 6-10 below 40 degrees C. Further, a novel electrochemical glucose biosensor was developed by construction of Nafion/GDH-bacteria/multiwalled-carbon-nanotube modified electrode. The as-prepared biosensor is linear with the concentration of D-glucose within the range of 50-800 mu M and a low detection limit of 4 mu M D-glucose (S/N=3). Excess saccharides including D-galactose, D-fructose, D-cellbiose, L-arabinose and D-sucrose, D-maltose, D-mannose and D-xylose as well as common interfering substances (acetaminophen, ascorbic acid and uric acid) did not affect the detection of D-glucose (0.1 mM). The proposed biosensor is stable, specific, reproducible, simple, rapid and cost-effective, which can be used for detection of real samples. It is envisioned that this GDH-bacteria will be found promising applications in biofuel cell, glucose detection and cofactor reproduction system. (C) 2013 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Glucose Dehydrogenase Displayed Bacteria Bacterial Surface Display Electrochemical Glucose Biosensor Bacterial Electrode Saccharide Analysis |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.bios.2013.01.050 |
| 关键词[WOS] | ICE NUCLEATION PROTEIN ; N-TERMINAL DOMAIN ; ELECTRON-TRANSFER ; ESCHERICHIA-COLI ; ORGANOPHOSPHORUS HYDROLASE ; BACILLUS-SUBTILIS ; OXIDASE ; IMMOBILIZATION ; ENZYMES ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS记录号 | WOS:000317888600004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1688 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266042, Peoples R China 4.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China 5.Monash Univ, Dept Chem Engn, Clayton, Vic 3800, Australia 6.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy |
| 推荐引用方式 GB/T 7714 | Liang, Bo,Li, Liang,Tang, Xiangliang,et al. Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor[J]. BIOSENSORS & BIOELECTRONICS,2013,45(2013):19-24. |
| APA | Liang, Bo.,Li, Liang.,Tang, Xiangliang.,Lang, Qiaolin.,Wang, Hongwei.,...&Liu, Aihua.(2013).Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor.BIOSENSORS & BIOELECTRONICS,45(2013),19-24. |
| MLA | Liang, Bo,et al."Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor".BIOSENSORS & BIOELECTRONICS 45.2013(2013):19-24. |
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