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Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell
Lang, Qiaolin1; Yin, Long1,3; Shi, Jianguo2; Li, Liang1; Xia, Lin1; Liu, Aihua1,3
2014-01-15
发表期刊BIOSENSORS & BIOELECTRONICS
卷号51期号:2014页码:158-163
摘要A novel electrochemical sequential biosensor was constructed by co-immobilizing glucoamylase (GA) and glucose oxidase (GOD) on the multi-walled carbon nanotubes (MWNTs)-modified glassy carbon electrode (GCE) by chemical crosslinking method, where glutaraldehyde and bovine serum albumin was used as crosslinking and blocking agent, respectively. The proposed biosensor (GA/GOD/MWNTs/GCE) is capable of determining starch without using extra sensors such as Clark-type oxygen sensor or H2O2 sensor. The current linearly decreased with the increasing concentration of starch ranging from 0.005% to 0.7% (w/w) with the limit of detection of 0.003% (w/w) starch. The as-fabricated sequential biosensor can be applicable to the detection of the content of starch in real samples, which are in good accordance with traditional Fehling's titration. Finally, a stable starch/O-2 biofuel cell was assembled using the GA/GOD/MWNTs/GCE as bioanode and laccase/MWNTs/GCE as biocathode, which exhibited open circuit voltage of ca. 0.53 V and the maximum power density of 8.15 mu W cm(-2) at 0.31 V, comparable with the other glucose/O-2 based biofuel cells reported recently. Therefore, the proposed biosensor exhibited attractive features such as good stability in weak acidic buffer, good operational stability, wide linear range and capable of determination of starch in real samples as well as optimal bioanode for the biofuel cell. (C) 2013 Elsevier B.V. All rights reserved.
文章类型Article
关键词Glucoamylase Glucose Oxidase Sequential Enzyme Biosensor Starch Biosensor Biofuel Cell
学科领域生物传感器技术
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2013.07.021
关键词[WOS]MICROBIAL FUEL-CELL ; GELATINIZATION ; SYSTEM ; MEMBRANES ; SUCROSE ; WORKING ; LACTOSE ; XYLOSE
收录类别SCI
语种英语
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000326905500022
引用统计
被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1684
专题生物传感技术团队(过去)
作者单位1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Bioenergy, Qingdao 266101, Peoples R China
2.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Lang, Qiaolin,Yin, Long,Shi, Jianguo,et al. Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell[J]. BIOSENSORS & BIOELECTRONICS,2014,51(2014):158-163.
APA Lang, Qiaolin,Yin, Long,Shi, Jianguo,Li, Liang,Xia, Lin,&Liu, Aihua.(2014).Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell.BIOSENSORS & BIOELECTRONICS,51(2014),158-163.
MLA Lang, Qiaolin,et al."Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell".BIOSENSORS & BIOELECTRONICS 51.2014(2014):158-163.
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