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Title:
Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell
Author: Lang, Qiaolin1; Yin, Long1,3; Shi, Jianguo2; Li, Liang1; Xia, Lin1; Liu, Aihua1,3
Source: BIOSENSORS & BIOELECTRONICS
Issued Date: 2014-01-15
Volume: 51, Issue:2014, Pages:158-163
Keyword: Glucoamylase ; Glucose oxidase ; Sequential enzyme biosensor ; Starch biosensor ; Biofuel cell
Subject: 生物资源::生物传感器技术
DOI: 10.1016/j.bios.2013.07.021
DOC Type: Article
English Abstract: A novel electrochemical sequential biosensor was constructed by co-immobilizing glucoamylase (GA) and glucose oxidase (GOD) on the multi-walled carbon nanotubes (MWNTs)-modified glassy carbon electrode (GCE) by chemical crosslinking method, where glutaraldehyde and bovine serum albumin was used as crosslinking and blocking agent, respectively. The proposed biosensor (GA/GOD/MWNTs/GCE) is capable of determining starch without using extra sensors such as Clark-type oxygen sensor or H2O2 sensor. The current linearly decreased with the increasing concentration of starch ranging from 0.005% to 0.7% (w/w) with the limit of detection of 0.003% (w/w) starch. The as-fabricated sequential biosensor can be applicable to the detection of the content of starch in real samples, which are in good accordance with traditional Fehling's titration. Finally, a stable starch/O-2 biofuel cell was assembled using the GA/GOD/MWNTs/GCE as bioanode and laccase/MWNTs/GCE as biocathode, which exhibited open circuit voltage of ca. 0.53 V and the maximum power density of 8.15 mu W cm(-2) at 0.31 V, comparable with the other glucose/O-2 based biofuel cells reported recently. Therefore, the proposed biosensor exhibited attractive features such as good stability in weak acidic buffer, good operational stability, wide linear range and capable of determination of starch in real samples as well as optimal bioanode for the biofuel cell. (C) 2013 Elsevier B.V. All rights reserved.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS Subject: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Subject Extended: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS Keyword Plus: MICROBIAL FUEL-CELL ; GELATINIZATION ; SYSTEM ; MEMBRANES ; SUCROSE ; WORKING ; LACTOSE ; XYLOSE
Indexed Type: SCI
Language: 英语
WOS ID: WOS:000326905500022
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/1684
Appears in Collections:生物传感技术团队_期刊论文

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description.institution: 1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Bioenergy, Qingdao 266101, Peoples R China
2.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Lang, Qiaolin,Yin, Long,Shi, Jianguo,et al. Co-immobilization of glucoamylase and glucose oxidase for electrochemical sequential enzyme electrode for starch biosensor and biofuel cell[J]. BIOSENSORS & BIOELECTRONICS,2014,51(2014):158-163.
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