| Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol | |
Jiang, Xinglin1,2; Zhang, Haibo1 ; Yang, Jianming1; Zheng, Yanning1,2; Feng, Dexin1; Liu, Wei1; Xu, Xin1; Cao, Yujin1; Zou, Huibin1; Zhang, Rubin1; Cheng, Tao1; Jiao, Fengjiao1; Xian, Mo1
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| 2013-08-15 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| 卷号 | 3期号:3页码:2445 |
| 摘要 | Methylacetoin (3-hydroxy-3-methylbutan-2-one) and 2-methyl-2,3-butanediol are currently obtained exclusively via chemical synthesis. Here, we report, to the best of our knowledge, the first alternative route, using engineered Escherichia coli. The biological synthesis of methylacetoin was first accomplished by reversing its biodegradation, which involved modifying the enzyme complex involved, switching the reaction substrate, and coupling the process to an exothermic reaction. 2-Methyl-2,3-butanediol was then obtained by reducing methylacetoin by exploiting the substrate promiscuity of acetoin reductase. A complete biosynthetic pathway from renewable glucose and acetone was then established and optimized via in vivo enzyme screening and host metabolic engineering, which led to titers of 3.4 and 3.2 g l(-1) for methylacetoin and 2-methyl-2,3-butanediol, respectively. This work presents a biodegradation-inspired approach to creating new biosynthetic pathways for small molecules with no available natural biosynthetic pathway. |
| 文章类型 | Article |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology |
| DOI | 10.1038/srep02445 |
| 关键词[WOS] | ESCHERICHIA-COLI ; ACETOIN ; METABOLISM ; DEHYDROGENASE ; KETONE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000323099700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1679 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Xinglin,Zhang, Haibo,Yang, Jianming,et al. Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol[J]. SCIENTIFIC REPORTS,2013,3(3):2445. |
| APA | Jiang, Xinglin.,Zhang, Haibo.,Yang, Jianming.,Zheng, Yanning.,Feng, Dexin.,...&Xian, Mo.(2013).Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol.SCIENTIFIC REPORTS,3(3),2445. |
| MLA | Jiang, Xinglin,et al."Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol".SCIENTIFIC REPORTS 3.3(2013):2445. |
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