Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli

doi:10.1016/j.biortech.2013.08.105

QIBEBT-IR > 生物基材料组群

	Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli
	Zou, Huibin; Wu, Zaiqiang; Xian, Mo; Liu, Hui; Cheng, Tao; Cao, Yujin
	2013-11-01
发表期刊	BIORESOURCE TECHNOLOGY
卷号	148 期号:148c 页码:591-595
摘要	Lactobacilli are commonly used for industrial production of polymer-grade L-lactic acid. The present study tested the Tween 80 alternative betaine in L-lactate production by several industrial lactobacilli. In flask fermentation of Lactobacillus casei, Lactobacillus buchneri, Lactobacillus lactis and Lactobacillus rhamnosus, the betaine addition (2 g/l) had similar osmoprotectant effect with Tween 80 but had increased the lactate dehydrogenase activities and L-lactate production than Tween 80 control. In fed-batch fermentation of L. casei, betaine supplementation improved the L-lactic acid titer to 190 g/l, the yield to 95.5% (g L-lactic acid/g glucose), the productivity to 2.6 g/l h, and the optical purity to 97.0%. The results demonstrated that supplementation of Tween 80 alternative - betaine in the fermentation medium is feasible for industrial L-lactic acid fermentation by lactobacilli, which will improve the lactate production but will not increase the process costs and modify any process conditions. (C) 2013 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	L-lactic Acid Lactobacilli Betaine Osmoprotectant Lactate Dehydrogenase Activity
学科领域	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1016/j.biortech.2013.08.105
关键词[WOS]	L-LACTIC ACID ; GLYCINE BETAINE ; EFFICIENT PRODUCTION ; BACILLUS-SUBTILIS ; GROWTH ; FERMENTATION ; MODULATION ; TOLERANCE ; SURVIVAL ; PURITY
收录类别	SCI
语种	英语
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000326905400080
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1678
专题	生物基材料组群
作者单位	Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Zou, Huibin,Wu, Zaiqiang,Xian, Mo,et al. Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli[J]. BIORESOURCE TECHNOLOGY,2013,148(148c):591-595.
APA	Zou, Huibin,Wu, Zaiqiang,Xian, Mo,Liu, Hui,Cheng, Tao,&Cao, Yujin.(2013).Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli.BIORESOURCE TECHNOLOGY,148(148c),591-595.
MLA	Zou, Huibin,et al."Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli".BIORESOURCE TECHNOLOGY 148.148c(2013):591-595.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Not only osmoprotect（715KB）			开放获取	CC BY-NC-SA	浏览下载