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	Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.
	Li, T.; Du, Y.; Cui, Q.; Zhang, J.; Zhu, W.; Hong, K.; & Li, W.
	2013
发表期刊	Mar Drugs
卷号	11期号:2页码:466-488
摘要	The indolocarbazole (ICZ) alkaloids have attracted much attention due to their unique structures and potential therapeutic applications. A series of ICZs were recently isolated and identified from a marine-derived actinomycete strain, Streptomyces sanyensis FMA. To elucidate the biosynthetic machinery associated with ICZs production in S. sanyensis FMA, PCR using degenerate primers was carried out to clone the FAD-dependent monooxygenase gene fragment for ICZ ring formation, which was used as a probe to isolate the 34.6-kb DNA region containing the spc gene cluster. Sequence analysis revealed genes for ICZ ring formation (spcO, D, P, C), sugar unit formation (spcA, B, E, K, J, I), glycosylation (spcN, G), methylation (spcMA, MB), as well as regulation (spcR). Their involvement in ICZ biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152. This work represents the first cloning and characterization of an ICZ gene cluster isolated from a marine-derived actinomycete strain and would be helpful for thoroughly understanding the biosynthetic mechanism of ICZ glycosides.
关键词	Indolocarbazole (Icz) Biosynthesis Heterologous Expression
学科领域	代谢物组学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1677
专题	代谢物组学研究组
推荐引用方式 GB/T 7714	Li, T.,Du, Y.,Cui, Q.,et al. Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.[J]. Mar Drugs,2013,11(2):466-488.
APA	Li, T..,Du, Y..,Cui, Q..,Zhang, J..,Zhu, W..,...&& Li, W..(2013).Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA..Mar Drugs,11(2),466-488.
MLA	Li, T.,et al."Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.".Mar Drugs 11.2(2013):466-488.

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