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	Separation and Quantification of Water-Soluble Cellular Metabolites inClostridium thermocellumusing Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry
	Cui, J.; Zhang, J.; Zhu, X.; Bai, F.; Feng, Y.; Guan, W.; & Cui, Q.
	2013
发表期刊	Analytical Letters
卷号	46期号:17页码:2767-2786
摘要	A new protocol for metabolomic studies was developed by combining liquid chromatographytandem mass spectrometry and isotope dilution mass spectrometry with universal 13C labeled internal standards from Escherichia Coli. The multiple reaction monitoring mode of mass spectrometry was used for quantification. Forty-five water-soluble intracellular metabolites, including 20 amino acids, 16 organic acids (primarily from the tricarboxylic acid cycle), and 9 cofactors, were measured and 34 of them were successfully quantified using the 13C-labeled internal standards. The limit of detection, limit of quantification, precision, and linearity of the methods were evaluated. The methods were applied to the quantitative analysis of intracellular metabolites extracted from wild-type and ethanol-adapted strains of Clostridium thermocellum cultivated with and without ethanol stress, and all 34 metabolites including all 9 cofactors were successfully quantified. Further multivariate data analyses of the metabolic differences between wild-type and ethanol-adapted strains were performed on the quantitative data, which can help elucidate the metabolic mechanism behind ethanol adaptation in C. thermocellum. Supplemental materials are available for this article. Go to the publisher’s onlineedition of Analytical Letters to view the supplemental file.
学科领域	代谢物组学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1671
专题	代谢物组学研究组
推荐引用方式 GB/T 7714	Cui, J.,Zhang, J.,Zhu, X.,et al. Separation and Quantification of Water-Soluble Cellular Metabolites inClostridium thermocellumusing Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry[J]. Analytical Letters,2013,46(17):2767-2786.
APA	Cui, J..,Zhang, J..,Zhu, X..,Bai, F..,Feng, Y..,...&& Cui, Q..(2013).Separation and Quantification of Water-Soluble Cellular Metabolites inClostridium thermocellumusing Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry.Analytical Letters,46(17),2767-2786.
MLA	Cui, J.,et al."Separation and Quantification of Water-Soluble Cellular Metabolites inClostridium thermocellumusing Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry".Analytical Letters 46.17(2013):2767-2786.

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