| Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli | |
Wang, Qi1,2; Yang, Peng1,3; Liu, Changshui1,2; Xue, Yongchang3; Xian, Mo1 ; Zhao, Guang1
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| 2013-03-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| 卷号 | 131期号:2013页码:548-551 |
| 摘要 | Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. After culture condition optimization, the final engineered strain accumulated 10.1 g/L P3HP (46.4% of the cell dry weight) using glycerol and glucose as cosubstrates in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without addition of any expensive precursor. (C) 2013 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Poly(3-hydroxypropionate) Glycerol Dehydratase Propionaldehyde Dehydrogenase Glycerol-glucose Cofermentation |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2013.01.096 |
| 关键词[WOS] | BACTERIAL POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYPROPIONATE) ; 3-HYDROXYPROPIONIC ACID ; REACTIVATING FACTOR ; DEHYDRATASE ; POLYESTERS ; MECHANISM ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000317024200074 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1669 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Yang, Peng,Liu, Changshui,et al. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2013,131(2013):548-551. |
| APA | Wang, Qi,Yang, Peng,Liu, Changshui,Xue, Yongchang,Xian, Mo,&Zhao, Guang.(2013).Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli.BIORESOURCE TECHNOLOGY,131(2013),548-551. |
| MLA | Wang, Qi,et al."Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli".BIORESOURCE TECHNOLOGY 131.2013(2013):548-551. |
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