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Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli
Wang, Qi1,2; Yang, Peng1,3; Liu, Changshui1,2; Xue, Yongchang3; Xian, Mo1; Zhao, Guang1
2013-03-01
发表期刊BIORESOURCE TECHNOLOGY
卷号131期号:2013页码:548-551
摘要Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. After culture condition optimization, the final engineered strain accumulated 10.1 g/L P3HP (46.4% of the cell dry weight) using glycerol and glucose as cosubstrates in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without addition of any expensive precursor. (C) 2013 Elsevier Ltd. All rights reserved.
文章类型Article
关键词Poly(3-hydroxypropionate) Glycerol Dehydratase Propionaldehyde Dehydrogenase Glycerol-glucose Cofermentation
学科领域生物基化学品
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2013.01.096
关键词[WOS]BACTERIAL POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYPROPIONATE) ; 3-HYDROXYPROPIONIC ACID ; REACTIVATING FACTOR ; DEHYDRATASE ; POLYESTERS ; MECHANISM ; PATHWAY
收录类别SCI
语种英语
WOS研究方向Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号WOS:000317024200074
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1669
专题生物基材料组群
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
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Wang, Qi,Yang, Peng,Liu, Changshui,et al. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2013,131(2013):548-551.
APA Wang, Qi,Yang, Peng,Liu, Changshui,Xue, Yongchang,Xian, Mo,&Zhao, Guang.(2013).Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli.BIORESOURCE TECHNOLOGY,131(2013),548-551.
MLA Wang, Qi,et al."Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli".BIORESOURCE TECHNOLOGY 131.2013(2013):548-551.
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