| Metabolic Engineering of Escherichia coli for the Production of Xylonate | |
Cao, Yujin; Xian, Mo ; Zou, Huibin; Zhang, Haibo
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| 2013-07-05 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 8期号:7页码:1-7 |
| 摘要 | Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far. In this study, the industrially important microorganism Escherichia coli was engineered to produce xylonate from xylose. Through the coexpression of a xylose dehydrogenase (xdh) and a xylonolactonase (xylC) from Caulobacter crescentus, the recombinant strain could convert 1 g/L xylose to 0.84 g/L xylonate and 0.10 g/L xylonolactone after being induced for 12 h. Furthermore, the competitive pathway for xylose catabolism in E. coli was blocked by disrupting two genes (xylA and xylB) encoding xylose isomerase and xylulose kinase. Under fed-batch conditions, the finally engineered strain produced up to 27.3 g/L xylonate and 1.7 g/L xylonolactone from 30 g/L xylose, about 88% of the theoretical yield. These results suggest that the engineered E. coli strain has a promising perspective for large-scale production of xylonate. |
| 文章类型 | Article |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0067305 |
| 关键词[WOS] | D-XYLOSE METABOLISM ; SACCHAROMYCES-CEREVISIAE ; PSEUDOMONAS-FRAGI ; ACID PRODUCTION ; DEHYDROGENASE ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000321425300010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1658 |
| 专题 | 生物基材料组群 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Yujin,Xian, Mo,Zou, Huibin,et al. Metabolic Engineering of Escherichia coli for the Production of Xylonate[J]. PLOS ONE,2013,8(7):1-7. |
| APA | Cao, Yujin,Xian, Mo,Zou, Huibin,&Zhang, Haibo.(2013).Metabolic Engineering of Escherichia coli for the Production of Xylonate.PLOS ONE,8(7),1-7. |
| MLA | Cao, Yujin,et al."Metabolic Engineering of Escherichia coli for the Production of Xylonate".PLOS ONE 8.7(2013):1-7. |
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| Metabolic Engineerin（2017KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
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