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Metabolic Engineering of Escherichia coli for the Production of Xylonate
Cao, Yujin; Xian, Mo; Zou, Huibin; Zhang, Haibo
2013-07-05
发表期刊PLOS ONE
卷号8期号:7页码:1-7
摘要Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far. In this study, the industrially important microorganism Escherichia coli was engineered to produce xylonate from xylose. Through the coexpression of a xylose dehydrogenase (xdh) and a xylonolactonase (xylC) from Caulobacter crescentus, the recombinant strain could convert 1 g/L xylose to 0.84 g/L xylonate and 0.10 g/L xylonolactone after being induced for 12 h. Furthermore, the competitive pathway for xylose catabolism in E. coli was blocked by disrupting two genes (xylA and xylB) encoding xylose isomerase and xylulose kinase. Under fed-batch conditions, the finally engineered strain produced up to 27.3 g/L xylonate and 1.7 g/L xylonolactone from 30 g/L xylose, about 88% of the theoretical yield. These results suggest that the engineered E. coli strain has a promising perspective for large-scale production of xylonate.
文章类型Article
学科领域生物基化学品
WOS标题词Science & Technology
DOI10.1371/journal.pone.0067305
关键词[WOS]D-XYLOSE METABOLISM ; SACCHAROMYCES-CEREVISIAE ; PSEUDOMONAS-FRAGI ; ACID PRODUCTION ; DEHYDROGENASE ; GENES
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000321425300010
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1658
专题生物基材料组群
作者单位Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China
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Cao, Yujin,Xian, Mo,Zou, Huibin,et al. Metabolic Engineering of Escherichia coli for the Production of Xylonate[J]. PLOS ONE,2013,8(7):1-7.
APA Cao, Yujin,Xian, Mo,Zou, Huibin,&Zhang, Haibo.(2013).Metabolic Engineering of Escherichia coli for the Production of Xylonate.PLOS ONE,8(7),1-7.
MLA Cao, Yujin,et al."Metabolic Engineering of Escherichia coli for the Production of Xylonate".PLOS ONE 8.7(2013):1-7.
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