| Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp. | |
Qi, Fengxia1,2 ; Yao, Lun1,2; Tan, Xiaoming1; Lu, Xuefeng1
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| 2013-10-01 | |
| 发表期刊 | BIOTECHNOLOGY LETTERS
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| 卷号 | 35期号:10页码:1655-1661 |
| 摘要 | An integrative gene expression system has been constructed for the directional assembly of biological components in Synechocystis PCC6803. We have characterized 11 promoter parts with various expression efficiencies for genetic engineering of Synechocystis for the production of fatty alcohols. This was achieved by integrating several genetic modifications including the expression of multiple-copies of fatty acyl-CoA reductase (FAR) under the control of strong promoters, disruption of the competing pathways for poly-beta-hydroxybutyrate and glycogen synthesis, and for peptide truncation of the FAR. In shake-flask cultures, the production of fatty alcohols was significantly improved with a yield of 761 +/- A 216 mu g/g cell dry weight in Synechocystis, which is the highest reported to date. |
| 文章类型 | Article |
| 关键词 | Fatty Alcohol Fatty Acyl-coa Reductase Promoters Synechocystis Sp Pcc6803 Synthetic Biology |
| 学科领域 | 生物代谢工程 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10529-013-1252-0 |
| 关键词[WOS] | EXPRESSION ; CYANOBACTERIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000325011900015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1635 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Fengxia,Yao, Lun,Tan, Xiaoming,et al. Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp.[J]. BIOTECHNOLOGY LETTERS,2013,35(10):1655-1661. |
| APA | Qi, Fengxia,Yao, Lun,Tan, Xiaoming,&Lu, Xuefeng.(2013).Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp..BIOTECHNOLOGY LETTERS,35(10),1655-1661. |
| MLA | Qi, Fengxia,et al."Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp.".BIOTECHNOLOGY LETTERS 35.10(2013):1655-1661. |
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