| Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method | |
| Song, Xiangfei1,2; Wang, Yefei1,2; Shu, Zhiyu1,2; Hong, Jingbo1,2; Li, Tong1,2; Yao, Lishan1,2 | |
| 2013-07-01 | |
| 发表期刊 | PLOS COMPUTATIONAL BIOLOGY
 |
| 卷号 | 9期号:7 |
| 摘要 | The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the Tm of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability. ; The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the T-m of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability. |
| 文章类型 | Article |
| 学科领域 | 仿真与模拟 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1371/journal.pcbi.1003129 |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; FREE-ENERGY CALCULATIONS ; CHARGE-CHARGE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR-DYNAMICS ; REPORTER PROTEINS ; T4 LYSOZYME ; STABILITY ; STABILIZATION ; SIMULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Mathematical & Computational Biology |
| WOS类目 | Biochemical Research Methods ; Mathematical & Computational Biology |
| WOS记录号 | WOS:000322320200017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1634 |
| 专题 | 蛋白质设计研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,et al. Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method[J]. PLOS COMPUTATIONAL BIOLOGY,2013,9(7). |
| APA | Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,Hong, Jingbo,Li, Tong,&Yao, Lishan.(2013).Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method.PLOS COMPUTATIONAL BIOLOGY,9(7). |
| MLA | Song, Xiangfei,et al."Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method".PLOS COMPUTATIONAL BIOLOGY 9.7(2013). |
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