| Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources | |
Yuan, Bo1,2; Wang, Shi-An1,3 ; Li, Fu-Li1,3
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| 2013-10-01 | |
| 发表期刊 | BIOTECHNOLOGY LETTERS
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| 卷号 | 35期号:10页码:1589-1592 |
| 摘要 | To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l-1 h-1) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l-1 h-1) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene; To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources. |
| 文章类型 | Article |
| 关键词 | Biofuel Ethanol Inulinase Jerusalem Artichoke Yeast |
| 学科领域 | 微生物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10529-013-1251-1 |
| 关键词[WOS] | JERUSALEM-ARTICHOKE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000325011900006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1624 |
| 专题 | 分子微生物工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Bo,Wang, Shi-An,Li, Fu-Li. Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources[J]. BIOTECHNOLOGY LETTERS,2013,35(10):1589-1592. |
| APA | Yuan, Bo,Wang, Shi-An,&Li, Fu-Li.(2013).Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.BIOTECHNOLOGY LETTERS,35(10),1589-1592. |
| MLA | Yuan, Bo,et al."Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources".BIOTECHNOLOGY LETTERS 35.10(2013):1589-1592. |
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